Roden Anja C, Lockington Karen S, Tostrud Linda J, Katzmann Jerry A
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Am J Clin Pathol. 2008 Jul;130(1):141-5. doi: 10.1309/6K33KTFA7A5VUQ1T.
Our objective was to evaluate a gel system that uses unconcentrated urine specimens for protein electrophoresis (PEL) and immunofixation electrophoresis (IFE) in patients with monoclonal gammopathies. For the study, 222 urine specimens were analyzed by our current PEL method (Helena Laboratories, Beaumont, TX) and by a system that recommends use of unconcentrated urine (Sebia, Norcross, GA). M protein concentrations were compared in the 43 cases with a measurable M spike. IFE was performed on 111 of the samples using both methods. There was a 97% concordance for detection of PEL abnormalities. The concordance for IFE was 98%. M protein concentrations by the 2 methods correlated well (r2=0.99; slope, 1.04). Cases with insufficient urine volumes for concentration (PEL, 7; IFE, 20) were analyzed in the Sebia gel system, and in 11 cases (PEL, 2; IFE, 9) an M protein was identified.High-resolution gel electrophoresis of urine using the Sebia system offers similar performance for detection, characterization, and quantification of M proteins when compared with our current gel system. Testing unconcentrated urine specimens will mean fewer sample rejections owing to insufficient sample volume.
我们的目的是评估一种凝胶系统，该系统可使用未浓缩的尿液标本对单克隆丙种球蛋白病患者进行蛋白质电泳（PEL）和免疫固定电泳（IFE）。在本研究中，我们采用当前的PEL方法（Helena Laboratories，博蒙特，德克萨斯州）和一种推荐使用未浓缩尿液的系统（Sebia，诺克罗斯，佐治亚州）对222份尿液标本进行了分析。对43例可检测到M峰的病例比较了M蛋白浓度。使用两种方法对111份样本进行了IFE检测。PEL异常检测的一致性为97%。IFE的一致性为98%。两种方法测得的M蛋白浓度相关性良好（r2=0.99；斜率，1.04）。对Sebia凝胶系统中尿液量不足无法浓缩的病例（PEL检测7例；IFE检测20例）进行了分析，其中11例（PEL检测2例；IFE检测9例）检测到M蛋白。与我们当前的凝胶系统相比，使用Sebia系统对尿液进行高分辨率凝胶电泳在检测、鉴定和定量M蛋白方面具有相似的性能。检测未浓缩的尿液标本意味着因样本量不足而导致的样本拒收情况会减少。
