Bertoli Alessandra, Giovannini Annalisa, Ruffoni Barbara, Guardo Angela Di, Spinelli Guido, Mazzetti Michele, Pistelli Luisa
Dipartimento di Chimica Bioorganica e Biofarmacia (DCBB), Università di Pisa, via Bonanno 33, 56126 Pisa, Italy.
J Agric Food Chem. 2008 Jul 9;56(13):5078-82. doi: 10.1021/jf0729107. Epub 2008 Jun 14.
A wild strain of Agrobacterium rhizogenes was used to regenerate twelve in vitro plant lines from different hairy roots of H. perforatum (St. John's Wort). The production of the main bioactive constituents was observed even though their yields varied in the different plant lines. Two lines were selected for the hyperoside production (4.9-4.6 mg/gdw) while nine were characterized by significant yields of chlorogenic acid (ranged from 0.47 to 1.09 mg/gdw). Furthermore, one out of twelve lines showed a 10-fold higher hypericin content (0.25 mg/gdw) than that reported for the in vitro shoots in the literature. Morphological and phytochemical features were determined in order to select H. perforatum genotypes enriched in valuable bioactive compounds.
使用发根农杆菌的野生菌株从贯叶连翘不同的毛状根中再生出12个离体植株系。尽管不同植株系中主要生物活性成分的产量有所不同,但仍观察到了它们的产生。选择了两个用于金丝桃苷生产的株系(4.9 - 4.6毫克/克干重),而九个株系的绿原酸产量显著(范围为0.47至1.09毫克/克干重)。此外,十二个株系中有一个的金丝桃素含量比文献中报道的离体芽高10倍(0.25毫克/克干重)。为了选择富含珍贵生物活性化合物的贯叶连翘基因型,对其形态和植物化学特征进行了测定。