Lu Y, Liu T, Li H, Pi G
Endodontic Department, Beijing Hospital of Stomatology, Capital University of Medical Science, Beijing, China.
Int Endod J. 2008 Aug;41(8):643-50. doi: 10.1111/j.1365-2591.2008.01396.x. Epub 2008 Jun 28.
To evaluate human pulp tissue response following direct pulp capping with a self-etching adhesive: Clearfil SE BOND (SB).
Forty-five sound teeth from 20 subjects were used. Forty-one teeth had their pulp mechanically exposed at the base of a Class 1 cavity preparation and were divided into two groups: group 1, teeth were capped with SB (n = 21), and group 2, with calcium hydroxide cement (CH) (n = 20). Four teeth were maintained intact as an untreated control group. After 7, 30 and 90 days, respectively, 15 teeth were extracted and processed for light microscopic examination. Pulp healing and bacterial microleakage were assessed by haematoxylin and eosin, Masson trichrome and Brown and Brenn stain techniques. The data were analysed statistically by using the Mann-Whitney U test.
After the 7-day observation period, the inflammatory reaction in the SB group was slight and significantly less severe than that of the CH group (P < 0.05). After the 30- and 90-day observation periods, the inflammatory reaction was slight in both groups, but specimens with dentine bridge formation in the SB group were significantly less common than those in the CH group (P < 0.05).
Clearfil SB had good biocompatibility with human pulp tissue, but its ability to induce reparative dentine was significantly lower than that of calcium hydroxide.
评估使用自酸蚀粘结剂Clearfil SE BOND（SB）进行直接盖髓后人体牙髓组织的反应。
使用来自20名受试者的45颗健康牙齿。41颗牙齿在Ⅰ类洞制备底部进行机械露髓，并分为两组：第1组，用SB覆盖牙齿（n = 21），第2组，用氢氧化钙水门汀（CH）覆盖（n = 20）。4颗牙齿保持完整作为未处理的对照组。分别在7天、30天和90天后，拔除15颗牙齿并进行处理以进行光学显微镜检查。通过苏木精和伊红染色、马松三色染色以及布朗和布伦染色技术评估牙髓愈合和细菌微渗漏情况。使用曼-惠特尼U检验对数据进行统计学分析。
在7天观察期后，SB组的炎症反应轻微，且明显不如CH组严重（P < 0.05）。在30天和90天观察期后，两组的炎症反应均轻微，但SB组形成牙本质桥的标本明显少于CH组（P < 0.05）。
Clearfil SB与人体牙髓组织具有良好的生物相容性，但其诱导修复性牙本质形成的能力明显低于氢氧化钙。
