Nordlinger B, Panis Y, Puts J P, Herve J P, Delelo R, Ballet F
INSERM U.181, Paris, France.
Dis Colon Rectum. 1991 Aug;34(8):658-63. doi: 10.1007/BF02050346.
The liver is the most frequent site of metastases in colon cancer. No good animal model has been available to help improve the treatment of liver metastases or their prevention after resection of a primary colon cancer. The aim of this study was to develop a model of colon cancer induced by azoxymethane in the rat and to study the outcome after surgical resection alone or in association with intraperitoneal chemotherapy (5-fluorouracil (5-FU). Three hundred male Wistar rats received subcutaneous azoxymethane (10 mg/kg body weight/week) for 12 weeks. Eighty-three rats with isolated colon cancer underwent total colectomy; 40 of these rats with no metastases were randomized into two groups: surgery alone or surgery plus 5-FU (5 mg/kg body weight/day) for 5 days after surgery. Thirty rats were able to be evaluated. At autopsy, peritoneal carcinomatosis and liver metastases were more frequent in the control group than after adjuvant treatment with 5-FU (27.7 percent vs. 0, P less than 0.05; and 22.2 percent vs. 0, P less than 0.05, respectively). The rates of peritoneal and hepatic recurrence after resection of the primary cancer indicate that the model mimics the natural history of human colon cancer. In this model, 5-FU reduced the rate of peritoneal carcinomatosis and liver metastases but did not influence survival.
肝脏是结肠癌最常见的转移部位。目前尚无良好的动物模型可用于帮助改善肝转移癌的治疗或预防原发性结肠癌切除术后的肝转移。本研究的目的是建立一种由氧化偶氮甲烷诱导的大鼠结肠癌模型，并研究单纯手术切除或联合腹腔化疗（5-氟尿嘧啶（5-FU））后的结果。300只雄性Wistar大鼠接受皮下注射氧化偶氮甲烷（10mg/kg体重/周），共12周。83只患有孤立性结肠癌的大鼠接受了全结肠切除术；其中40只无转移的大鼠被随机分为两组：单纯手术组或手术后5天接受5-FU（5mg/kg体重/天）治疗的手术加5-FU组。30只大鼠能够进行评估。尸检时，对照组的腹膜癌和肝转移比接受5-FU辅助治疗后更常见（分别为27.7%对0，P<0.05；22.2%对0，P<0.05）。原发性癌切除术后腹膜和肝脏复发率表明该模型模拟了人类结肠癌的自然病程。在该模型中，5-FU降低了腹膜癌和肝转移的发生率，但不影响生存率。
