de Paula P R, Speranzini M B, Hamzagic H C, Bassi D G, Chacon-Silva M A, Novo N F, Goldenberg S
Paulista Medical School, São Paulo, Brazil.
Dis Colon Rectum. 1991 Aug;34(8):664-9. doi: 10.1007/BF02050347.
The purpose of this study is to analyze the size of the bacterial colonies in anal wounds after open hemorrhoidectomy. Twenty patients were studied during predetermined postoperative time periods. Material was collected from the surface and from within the tissue of each patient's three open wounds, intraoperatively, on the 6th, 13th and 20th postoperative days for bacteriologic examination in aerobic, microaerophilic, and anaerobic media. The bacterium most commonly identified was Escherichia coli, followed by Staphylococcus aureus and Staphylococcus epidermidis. Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella pneumoniae, Proteus vulgaris, and Proteus mirabilis were also identified. Critical indexes of colonization were present since the intraoperative stage (greater than 10(5) bacteria/g of tissue and greater than 10(6) bacteria/ml); obligate anaerobic bacteria were not identified; neither the species nor the number of bacteria, even when critical indexes were present, prevented proper healing. The same bacteria were not necessarily present on the surface and in the tissue; the bacterial load observed among the three wounds (left lateral, right posterior, and right anterior), was the same.
本研究的目的是分析开放式痔切除术后肛门伤口中细菌菌落的大小。在预定的术后时间段内对20名患者进行了研究。在术中、术后第6天、第13天和第20天，从每位患者的三个开放伤口的表面和组织内采集材料，在需氧、微需氧和厌氧培养基中进行细菌学检查。最常鉴定出的细菌是大肠埃希菌，其次是金黄色葡萄球菌和表皮葡萄球菌。还鉴定出了铜绿假单胞菌、粪肠球菌、肺炎克雷伯菌、普通变形杆菌和奇异变形杆菌。自术中阶段起就存在定植的关键指标（组织中细菌数量大于10⁵/g，液体中细菌数量大于10⁶/ml）；未鉴定出专性厌氧菌；即使存在关键指标，细菌的种类和数量均未妨碍伤口正常愈合。表面和组织中不一定存在相同的细菌；在三个伤口（左侧、右后侧和右前侧）中观察到的细菌载量相同。
