采用多重聚合酶链反应对从火鸡肉中分离出的产气荚膜梭菌进行分子分型。
Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR.
作者信息
Erol I, Goncuoglu M, Ayaz N D, Bilir Ormanci F S, Hildebrandt G
机构信息
Department of Food Hygiene and Technology, School of Veterinary Medicine, Ankara University, Ankara, Turkey.
出版信息
Lett Appl Microbiol. 2008 Jul;47(1):31-4. doi: 10.1111/j.1472-765X.2008.02379.x. Epub 2008 Jun 12.
AIMS
To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing.
METHODS AND RESULTS
For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. perfringens isolates were found to carry the cpa, gene but in none of the isolates cpb, etx, iap or cpe genes were detected. Results showed that all isolates represented type A and were cpe negative.
CONCLUSIONS
Our results indicate that Cl. perfringens type A is the most common type in turkey meat. Also multiplex PCR is effective and rapid method for typing of Cl. perfringens.
SIGNIFICANCE AND IMPACT OF THE STUDY
It is the first study about molecular typing of Cl. perfringens using multiplex PCR in turkey meat samples in Turkey.
目的
通过分子分型确定从火鸡肉样品中分离出的22株产气荚膜梭菌中是否存在毒素基因。
方法与结果
为此，采用多重聚合酶链反应（PCR）分析α（cpa）、β（cpb）、β2（cpb2）、ε（etx）、ι（iap）和肠毒素（cpe）毒素基因。发现所有22株火鸡肉产气荚膜梭菌分离株均携带cpa基因，但未检测到任何分离株携带cpb、etx、iap或cpe基因。结果表明，所有分离株均为A型且cpe阴性。
结论
我们的结果表明，A型产气荚膜梭菌是火鸡肉中最常见的类型。此外，多重PCR是产气荚膜梭菌分型的有效且快速的方法。
研究的意义与影响
这是土耳其首次关于在火鸡肉样品中使用多重PCR对产气荚膜梭菌进行分子分型的研究。
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