Jee Byung Chul, Chen Hai-Ying, Chian Ri-Cheng
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea.
Fertil Steril. 2009 May;91(5 Suppl):2037-42. doi: 10.1016/j.fertnstert.2008.03.041. Epub 2008 Jun 12.
To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development.
Animal study.
University laboratory.
ANIMAL(S): CD-1 mice.
INTERVENTION(S): Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium.
MAIN OUTCOME MEASURE(S): Oocyte maturation, fertilization, and early embryonic development in vitro.
RESULT(S): After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls.
CONCLUSION(S): Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.
研究卵母细胞成熟培养基中的3型磷酸二酯酶抑制剂对后续胚胎发育的影响。
动物研究。
大学实验室。
CD-1小鼠。
将未成熟卵母细胞在含有特定3型磷酸二酯酶抑制剂西洛酰胺（1微摩尔/升、5微摩尔/升和10微摩尔/升）的培养基中减数分裂阻滞6小时和24小时进行早熟培养，然后在无抑制剂的培养基中进行体外成熟培养。
卵母细胞成熟、受精及体外早期胚胎发育情况。
用1微摩尔/升、5微摩尔/升和10微摩尔/升西洛酰胺进行6小时和24小时早熟培养后，与未阻滞的对照组相比，在成熟、受精及早期胚胎发育方面无差异。
用西洛酰胺进行6小时或24小时的早熟培养可能对未成熟小鼠卵母细胞的后续胚胎发育无益处。
