Li Guang-Peng, Yang Shufen, Liu Ying, Sessions Benjamin R, White Kenneth L, Bunch Thomas D
Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, People's Republic of China.
Fertil Steril. 2009 Aug;92(2):798-805. doi: 10.1016/j.fertnstert.2008.07.1702. Epub 2008 Sep 14.
To investigate the effects of nicotine in combination with okadaic acid (OA) or taxol on bovine oocyte maturation and subsequent embryonic development.
Prospective randomized study.
University research laboratory.
PATIENT(S): Bovine ovaries, oocytes, and embryos.
INTERVENTION(S): Oocyte maturation and subsequent embryo development in the presence of nicotine in combination with okadaic acid and taxol.
MAIN OUTCOME MEASURE(S): Haploid composition, cleavage rate, IVF and parthenogenetic embryo development, blastocyst cell number.
RESULT(S): The results showed that nicotine and OA or nicotine and taxol significantly decreased oocyte maturation rates. Combinations of nicotine (10 or 50 micromol/L) and OA (0.01 or 0.05 micromol/L) did not affect oocyte haploid composition; however, taxol alone or combined with nicotine caused a decrease in haploid composition compared with control. Parthenogenetic activation of oocytes that were matured in nicotine, OA, or taxol resulted in blastocyst development rates of 12%-17%, which were not different from the control. Various combinations of nicotine and OA or nicotine and taxol significantly lowered (3%-8%) blastocyst development compared with control. The average cell number of blastocysts derived from nicotine + OA- and nicotine + taxol-treated oocytes was lower than all other treatment groups and control. For oocytes fertilized in vitro, oocytes matured in OA, taxol, or combinations of nicotine and OA or taxol-containing media resulted in significantly lower cleavage rates and blastocyst development compared with the control group and the 10 micromol/L nicotine treatment group. The IVF embryos cultured in nicotine + OA-containing medium had a significantly lower blastocyst development rate.
CONCLUSION(S): Combinations of nicotine with OA or taxol adversely affect oocyte maturation and subsequently result in poor blastocyst development.
研究尼古丁与冈田酸（OA）或紫杉醇联合使用对牛卵母细胞成熟及后续胚胎发育的影响。
前瞻性随机研究。
大学研究实验室。
牛卵巢、卵母细胞和胚胎。
在存在尼古丁与冈田酸及紫杉醇的情况下进行卵母细胞成熟及后续胚胎发育。
单倍体组成、卵裂率、体外受精和孤雌胚胎发育、囊胚细胞数。
结果显示，尼古丁与OA或尼古丁与紫杉醇显著降低卵母细胞成熟率。尼古丁（10或50微摩尔/升）与OA（0.01或0.05微摩尔/升）联合使用不影响卵母细胞单倍体组成；然而，单独使用紫杉醇或与尼古丁联合使用导致单倍体组成较对照组降低。在尼古丁、OA或紫杉醇中成熟的卵母细胞孤雌激活后，囊胚发育率为12%-17%，与对照组无差异。尼古丁与OA或尼古丁与紫杉醇的各种联合使用与对照组相比显著降低（3%-8%）囊胚发育。来自尼古丁+OA处理组和尼古丁+紫杉醇处理组卵母细胞的囊胚平均细胞数低于所有其他处理组和对照组。对于体外受精的卵母细胞，在OA、紫杉醇或含尼古丁与OA或紫杉醇的培养基中成熟的卵母细胞与对照组和10微摩尔/升尼古丁处理组相比，卵裂率和囊胚发育显著降低。在含尼古丁+OA培养基中培养的体外受精胚胎囊胚发育率显著降低。
尼古丁与OA或紫杉醇联合使用对卵母细胞成熟有不利影响，随后导致囊胚发育不良。
