Kohri K, Kodama M, Ishikawa Y, Katayama Y, Matsuda H, Imanishi M, Takada M, Katoh Y, Kataoka K, Akiyama T
Department of Urology, Kinki University School of Medicine, Osaka, Japan.
Eur Urol. 1991;19(3):249-52. doi: 10.1159/000473631.
The interaction of calcium oxalate crystals and renal tubular cells was studied. Rat renal collecting tubular cells were cultured and immunologically stained with anti-type-IV collagen antiserum (type-IV collagen exists in renal tubular basement membrane). When renal tubular cells and calcium oxalate crystals were mixed, clumps were formed. These clumps were examined by immunological staining with anti-type-IV collagen antiserum. In another series of experiments, calcium-containing crystals were found to be adsorbed onto mucous threads and cast-like materials, although no such adsorption was observed on squamous cells. These absorbed materials interacted with anti-type-IV collagen antiserum. These results suggest that collagen in the renal tubular basement membrane may act as matrix in urinary stone formation.
研究了草酸钙晶体与肾小管细胞的相互作用。培养大鼠肾集合管细胞，并用抗IV型胶原抗血清进行免疫染色（IV型胶原存在于肾小管基底膜中）。当肾小管细胞与草酸钙晶体混合时，会形成团块。用抗IV型胶原抗血清对这些团块进行免疫染色检查。在另一系列实验中，发现含钙晶体吸附在黏液丝和铸型样物质上，而在鳞状细胞上未观察到这种吸附现象。这些吸附物质与抗IV型胶原抗血清发生相互作用。这些结果表明，肾小管基底膜中的胶原可能在尿路结石形成中起基质作用。
