Vrensen G F, Graw J, De Wolf A
Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
Exp Eye Res. 1991 Jun;52(6):647-59. doi: 10.1016/0014-4835(91)90017-9.
The pre and post-natal development of wild type mouse lenses was studied by transmission electron microscopy, with special emphasis on denucleation of primary lens fibres. Denucleation of primary fibres is characterized by nuclear accumulation of small granules, most likely nucleosomes, which are condensed to osmiophilic bodies in the nucleus and in the cytoplasm. The osmiophilic bodies are laid down in apposition to the fibre membrane and are invaded by vesicles and granules, which probably contain proteolytic enzymes. Part of the breakdown products are extruded into the extracellular space, transported to the anterior and posterior poles where they might be finally digested or discarded from the lens. The morphology of the denucleation process of primary fibres is different from the gradual fading of nuclei in secondary fibres as described by Kuwabara and Imaizumi (1974: Invest. Ophthalmol. Vis. Sci. 13, 973-81).
通过透射电子显微镜研究了野生型小鼠晶状体的产前和产后发育，特别着重于初级晶状体纤维的去核过程。初级纤维的去核特征是小颗粒（很可能是核小体）在细胞核内积累，这些小颗粒在细胞核和细胞质中浓缩为嗜锇小体。嗜锇小体紧贴纤维膜沉积，并被可能含有蛋白水解酶的小泡和颗粒侵入。部分分解产物被挤出到细胞外空间，运输到晶状体的前后极，在那里它们最终可能被消化或从晶状体中丢弃。初级纤维去核过程的形态与Kuwabara和Imaizumi（1974年：《Invest. Ophthalmol. Vis. Sci.》13卷，973 - 981页）所描述的次级纤维中细胞核逐渐消退的情况不同。
