Gales Grégoire, Penninckx Michel, Block Jean-Claude, Leroy Pierre
Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Faculté de Pharmacie, CNRS Nancy-University, Nancy Cedex, France.
FEMS Yeast Res. 2008 Aug;8(5):667-75. doi: 10.1111/j.1567-1364.2008.00401.x. Epub 2008 Jun 28.
The resistance of Saccharomyces cerevisiae to oxidative stress (H(2)O(2) and Cd(2+)) was compared in biofilms and planktonic cells, with the help of yeast mutants deleted of genes related to glutathione metabolism and oxidative stress. Biofilm-forming cells were found predominantly in the G1 stage of the cell cycle. This might explain their higher tolerance to oxidative stress and the young replicative age of these cells in an old culture. The reduced glutathione status of S. cerevisiae was affected by the growth phase and apparently plays an important role in oxidative stress tolerance in cells growing as a biofilm.
借助缺失与谷胱甘肽代谢和氧化应激相关基因的酵母突变体，比较了酿酒酵母生物膜细胞和浮游细胞对氧化应激（H₂O₂和Cd²⁺）的抗性。发现形成生物膜的细胞主要处于细胞周期的G1期。这可能解释了它们对氧化应激具有更高的耐受性，以及在老化培养物中这些细胞的年轻复制年龄。酿酒酵母的还原型谷胱甘肽状态受生长阶段的影响，并且显然在作为生物膜生长的细胞的氧化应激耐受性中起重要作用。
