Nimkerdphol Kittiwann, Nakagawa Masahiro
Department of Electrical Engineering, Faculty of Engineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
J Biosci Bioeng. 2008 May;105(5):486-92. doi: 10.1263/jbb.105.486.
The behavioral responses of zebrafish (Danio rerio) to sublethal concentrations of sodium hypochlorite (NaClO)-based household bleach were quantified in order to index toxicity of the solution. The swimming behavior of zebrafish was captured using nonplanar 3D stereocameras in combination with 3D coordinate computation with perspective correction (3DCCPC) to compute for actual 3D coordinates. Swimming trajectory and velocity were quantified by fractal dimension analysis. The results showed that, under incremental concentrations of NaClO-based aqueous solutions with the maximum of 0.005% v/v, the fractal dimension of swimming velocity trended to increase with the concentration. The fractal dimension of swimming trajectories trended to increase with pH. Hence, the results have proven that the system is a useful tool to indicate behavioral changes, which may be implemented in biomonitoring systems for acute toxicity bioassay.
为了测定基于次氯酸钠（NaClO）的家用漂白剂溶液的毒性，对斑马鱼（Danio rerio）在亚致死浓度下的行为反应进行了量化。使用非平面3D立体相机结合带有透视校正的3D坐标计算（3DCCPC）来捕捉斑马鱼的游泳行为，以计算实际的3D坐标。通过分形维数分析对游泳轨迹和速度进行量化。结果表明，在浓度最高为0.005% v/v的基于NaClO的水溶液浓度递增的情况下，游泳速度的分形维数随浓度增加而呈上升趋势。游泳轨迹的分形维数随pH值增加而呈上升趋势。因此，结果证明该系统是一种指示行为变化的有用工具，可用于生物监测系统中的急性毒性生物测定。
