Stevens N T, Tharmabala M, Dillane T, Greene C M, O'Gara J P, Humphreys H
Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Education & Research Centre, Beaumont Hospital, Dublin, Ireland.
Clin Microbiol Infect. 2008 Jul;14(7):719-22. doi: 10.1111/j.1469-0691.2008.02012.x.
Fifty-five Staphylococcus epidermidis isolates, classified as contaminants or causing device-related meningitis, from external ventricular drain (EVD) and non-EVD cerebrospinal fluid specimens were characterized. Thirty-three of 42 (78.6%) meningitis isolates were PCR-positive for ica and aap, known determinants of polysaccharide- and protein-mediated biofilm production, whereas five of 13 (38.5%) contaminants were ica- and aap-negative; 71.4% of meningitis isolates and 84.6% of contaminants produced biofilm. ica+aap+ meningitis isolates produced more biofilm than ica+aap- isolates (p 0.0020). ica+aap- isolates did not produce more biofilm than ica-aap+ isolates (p 0.4368). Apparently, ica and aap are associated with biofilm production in S. epidermidis device-related meningitis isolates.
对从外部脑室引流管（EVD）和非EVD脑脊液标本中分离出的55株表皮葡萄球菌进行了鉴定，这些菌株被归类为污染物或与器械相关的脑膜炎致病菌。42株脑膜炎分离株中有33株（78.6%）ica和aap的PCR检测呈阳性，ica和aap是多糖和蛋白质介导生物膜形成的已知决定因素，而13株污染物中有5株（38.5%）ica和aap呈阴性；71.4%的脑膜炎分离株和84.6%的污染物产生生物膜。ica+aap+脑膜炎分离株比ica+aap-分离株产生更多生物膜（p = 0.0020）。ica+aap-分离株产生的生物膜不比ica-aap+分离株多（p = 0.4368）。显然，ica和aap与表皮葡萄球菌器械相关脑膜炎分离株中的生物膜形成有关。
