Association between biofilm formation, structure and antibiotic resistance in isolated from neonatal septicemia in southwest Iran.

has emerged as the pathogen from neonatal septicemia. Antibiotic resistance and the capability of biofilm formation make these infections much harder to treat. Hence, the aim of this study was to investigate the association between biofilm formation, structure and antibiotic resistance in isolated from neonatal septicemia. Overall, 65 isolates were recovered from blood cultures of neonatal septicemia. Antibiotic resistance pattern and the biofilm production were determined using phenotypic methods. The presence of operon, the , the genes and SCC types were screened using PCR. Most isolates were resistant to erythromycin, while all isolates were sensitive to linezolid and vancomycin. Fifty-three percent of isolates were resistant to methicillin. SCC types II was found commonly among methicillin-resistant (MRSE) strains. The biofilm formation was observed in 65% of S.epidermidis isolates and the majority have polysaccharide matrix. and genes were found in 40% and 19% of isolates. Twenty-three isolates (62%) produced dissolvable polysaccharide intercellular adhesion (PIA)-dependent biofilms in SM after growth in TSB with NaCl and 14 (37%) isolates produced dissolvable protein-dependent biofilms in PK after growth in TSB with glucose. Three isolates (62%) produced dissolvable polysaccharide intercellular adhesion. Our data indicate the high rates of antibiotic resistance and the capability of biofilm formation among isolates. Hence, the transmission of these strains can cause an increased risk of serious nosocomial infections.