Al-Saeedi Fatma J
Nuclear Medicine Department, Faculty of Medicine, Kuwait University, Kuwait.
BMC Med Phys. 2008 Mar 13;8:1. doi: 10.1186/1756-6649-8-1.
99mTc-HMPAO is a well-established isotope useful in the detection of regional cerebral blood flow. Diabetes gives rise to arterial atherosclerotic changes that can lead to significant end organ dysfunction, prominently affecting perfusion to the heart, kidneys, eyes and brain. In the current study, we investigated the role of 99mTc-HMPAO cerebral perfusion scans in detecting early vascular changes in the diabetic brain.
Cerebral perfusion studies were performed on both control and streptozotocin-(STZ) induced diabetic male Wistar rats. Rat brain imaging using a gamma camera was performed for each group 0.5, 2, 4, and 24 hours post 99mTc-HMPAO injection. Data processing for each cerebral perfusion scan was performed by drawing a region of interest (ROI) circumferentially around the brain (B). Background (BKG) due to signal from the soft tissue of each rat was subtracted. Brain 99mTc-HMPAO uptake minus background counts (net brain counts; NBC) were then compared between the two groups.
The NBC (mean +/- SD) for the STZ group were statistically significantly higher (p = 0.0004) than those of the control group at each of the time points studied.
99mTc-HMPAO brain scan may be useful in the detection of early atherosclerotic changes in the diabetic rat brain.
99mTc-HMPAO是一种成熟的同位素，可用于检测局部脑血流量。糖尿病会引发动脉粥样硬化改变，进而导致重要终末器官功能障碍，显著影响心脏、肾脏、眼睛和大脑的灌注。在本研究中，我们调查了99mTc-HMPAO脑灌注扫描在检测糖尿病脑早期血管变化中的作用。
对对照组和链脲佐菌素（STZ）诱导的糖尿病雄性Wistar大鼠进行脑灌注研究。在注射99mTc-HMPAO后0.5、2、4和24小时，使用γ相机对每组大鼠进行脑成像。通过在脑周围圆周绘制感兴趣区域（ROI）对每次脑灌注扫描进行数据处理。减去每只大鼠软组织信号产生的背景（BKG）。然后比较两组之间脑99mTc-HMPAO摄取量减去背景计数（净脑计数；NBC）。
在每个研究时间点，STZ组的NBC（平均值±标准差）均显著高于对照组（p = 0.0004）。
99mTc-HMPAO脑扫描可能有助于检测糖尿病大鼠脑的早期动脉粥样硬化变化。
