Meyer D H, Sreenivasan P K, Fives-Taylor P M
Department of Microbiology and Molecular Genetics, College of Medicine, University of Vermont, Burlington 05405.
Infect Immun. 1991 Aug;59(8):2719-26. doi: 10.1128/iai.59.8.2719-2726.1991.
Actinobacillus actinomycetemcomitans, an oral bacterial species associated with periodontal disease, was found to invade human cell lines. Invasion was demonstrated by recovery of viable organisms from gentamicin-treated KB cell monolayers and by light and electron microscopy. Internalization occurred through a cytochalasin D-sensitive process. Invasion efficiencies of some A. actinomycetemcomitans strains were comparable to those of invasive members of the family Enterobacteriaceae. Differences in invasiveness were correlated with bacterial colonial morphology. Smooth variants invaded more proficiently than rough variants. A. actinomycetemcomitans can undergo a smooth-to-rough colonial morphology shift which results in the loss of invasiveness. Coordinated regulation of genes involved in the rough-to-smooth phenotypic transitions may play a role in the episodic nature of periodontal disease.
伴放线放线杆菌是一种与牙周疾病相关的口腔细菌，已发现它能侵入人类细胞系。通过从经庆大霉素处理的KB细胞单层中回收活的微生物以及通过光学显微镜和电子显微镜观察，证实了这种侵入现象。内化过程是通过一种对细胞松弛素D敏感的机制发生的。一些伴放线放线杆菌菌株的侵入效率与肠杆菌科的侵入性成员相当。侵入性的差异与细菌菌落形态有关。光滑型变体比粗糙型变体更易侵入。伴放线放线杆菌可发生从光滑型到粗糙型的菌落形态转变，这会导致侵入性丧失。参与粗糙型到光滑型表型转变的基因的协同调控可能在牙周疾病的发作特性中起作用。
