Mazouni C, Arun B, André F, Ayers M, Krishnamurthy S, Wang B, Hortobagyi G N, Buzdar A U, Pusztai L
Laboratoire de transfert biologique et oncologique, Marseille University, Houston, TX, USA.
Br J Cancer. 2008 Jul 8;99(1):68-71. doi: 10.1038/sj.bjc.6604443. Epub 2008 Jun 17.
Collagen IV is a major component of the vascular basement membrane and may be a marker of angiogenesis. Serum levels of this protein are elevated in some human cancers. Our objectives were to compare collagen IV levels in the serum of breast cancer patients and healthy women and to examine changes during preoperative chemotherapy. Sera from 51 patients with stage II-III breast cancer and 55 healthy controls were analysed. Collagen IV level was measured by a commercially available sandwich enzyme link immunoassay. Baseline serum levels were compared between cancer patients and healthy women and paired pre- and post-chemotherapy measurements were also performed in 39 patients who received preoperative chemotherapy and were correlated with response to therapy. The median serum collagen IV concentration was significantly higher in cancer patients (166 microg l(-1)) than in healthy women (115 microg l(-1)), P<0.001. Chemotherapy induced a significant further increase in serum collagen IV (167 microg l(-1) prechemo vs 206 microg l(-1) postchemo, P=0.001). There were no correlations between baseline collagen IV levels and response to therapy, age, clinical stage or HER2 status. In conclusion, patients with breast cancer have elevated levels of collagen IV compared to healthy women and collagen IV levels increase further during chemotherapy.
IV型胶原是血管基底膜的主要成分，可能是血管生成的一个标志物。在一些人类癌症中，这种蛋白质的血清水平会升高。我们的目标是比较乳腺癌患者和健康女性血清中的IV型胶原水平，并检查术前化疗期间的变化。分析了51例II - III期乳腺癌患者和55例健康对照者的血清。通过市售的夹心酶联免疫测定法测量IV型胶原水平。比较了癌症患者和健康女性的基线血清水平，并对39例接受术前化疗的患者进行了化疗前后的配对测量，并将其与治疗反应相关联。癌症患者血清IV型胶原浓度中位数（166μg l(-1)）显著高于健康女性（115μg l(-1)），P<0.001。化疗导致血清IV型胶原进一步显著升高（化疗前167μg l(-1) 对比化疗后206μg l(-1)，P = 0.001）。基线IV型胶原水平与治疗反应、年龄、临床分期或HER2状态之间无相关性。总之，与健康女性相比，乳腺癌患者的IV型胶原水平升高，且化疗期间IV型胶原水平进一步升高。
