Hong W S, Hong S I, Park S Y, Son Y, Lee Y S, Chung Y H, Yang S K, Suh D J, Min Y I
Department of Internal Medicine, Asan Medical Center, University of Ulsan, Songpa-ku, Seoul, Korea.
Anticancer Res. 1995 Nov-Dec;15(6B):2777-80.
Studies on the level of serum type IV collagen (IV C) have usually been focused on the disease with diffuse hepatic fibrosis. To investigate whether serum level of IV C was predictive for the development of liver cancer as well as liver cirrhosis, serum IV C level was measured by a one-step sandwich enzyme immunoassay. The mean level of serum IV C was 73.3 +/- 31.3 ng/ml in 48 controls. The levels (ng/ml) of IV C were 396.4 +/- 254.9, 429.6 +/- 320.7, 420.6 +/- 322.8, and 362.9 +/- 247.4 respectively in 11 patients with chronic hepatitis, 11 with liver cirrhosis, 16 with hepatocellular carcinoma (HCC) with cirrhosis, 10 with HCC without cirrhosis, and 10 with metasatic liver cancer, which were significantly higher than that in controls (p < 0.05). Serum IV C levels were also evaluated using a cut-off value which was determined as the mean plus two standard deviations in the controls, 136 ng/ml. The elevations above the cut-off value were observed in 91, 100, 80, and 90% respectively of 11 patients with cirrhosis, 16 with HCC with cirrhosis, 10 with HCC without cirrhosis, and 10 with metastatic liver cancer, while only one (9%) of 11 chronic hepatitis patients and none (0%) of 48 controls had elevated levels. The levels of serum IV C were analysed with regard to age, sex, serum levels of albumin, globulin, transaminases, alpha-fetoprotein, and diameter of liver mass, a significant difference being observed only between the diameter of HCC and serum level of IV C (p < 0.01). These results indicate that the measurement of serum IV C is a useful for the determination of primary and metastatic liver cancer as well as liver cirrhosis.
血清IV型胶原（IV C）水平的研究通常集中于弥漫性肝纤维化疾病。为了研究血清IV C水平是否可预测肝癌以及肝硬化的发生，采用一步夹心酶免疫测定法检测血清IV C水平。48名对照组的血清IV C平均水平为73.3±31.3 ng/ml。11例慢性肝炎患者、11例肝硬化患者、16例伴有肝硬化的肝细胞癌（HCC）患者、10例不伴有肝硬化的HCC患者以及10例转移性肝癌患者的IV C水平（ng/ml）分别为396.4±254.9、429.6±320.7、420.6±322.8和362.9±247.4，均显著高于对照组（p<0.05）。血清IV C水平也采用一个临界值进行评估，该临界值确定为对照组平均值加两个标准差，即136 ng/ml。11例肝硬化患者、16例伴有肝硬化的HCC患者、10例不伴有肝硬化的HCC患者以及10例转移性肝癌患者中分别有91%、100%、80%和90%的患者高于临界值，而11例慢性肝炎患者中只有1例（9%）高于临界值，48名对照组患者均无升高（0%）。分析血清IV C水平与年龄、性别、血清白蛋白、球蛋白、转氨酶、甲胎蛋白水平以及肝肿块直径的关系，仅观察到HCC直径与血清IV C水平之间存在显著差异（p<0.01）。这些结果表明，检测血清IV C对原发性和转移性肝癌以及肝硬化的诊断有一定帮助。
