Du Ying, Liu Mei-Ling, Jia Meng-Chun
Peking Union Medical College Postgraduate School, Beijing 100730, China.
Sheng Li Xue Bao. 2008 Jun 25;60(3):382-90.
A gene that could be potentially involved in spermatogenesis was identified and characterized by using suppression subtractive hybridization (SSH) and rapid amplification of cDNA ends (RACE) with total RNA from type A spermatogonia and pachytene spermatocytes of rat. This gene consists of 3 433 base pairs (bp) with a complete open reading frame (ORF) of 3 171 bp and encodes a putative protein containing 1057 amino acids. The nucleotide sequence displays a 93% identity to mouse ubiquitin-activating enzyme E1, Chr Y 1 (Ube1y1) and an 82% identity to human ubiquitin-activating enzyme E1 (UBE1). The putative protein of this gene contains an ubiquitin-activating enzyme signature 1 and an ubiquitin-activating enzyme active site, which are also existed in mouse ubiquitin-activating enzyme E1, human ubiquitin-activating enzyme E1 et al. So we named this gene as Rattus norvegicus ubiquitin-activating enzyme E1 (Ube1). The sequence of Ube1 was submitted to GenBank and the accession number is EF690356. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that Ube1 was specifically expressed in testis, while its expression was not detected in heart, brain, spleen, lung, liver, muscle, kidney and ovary. Comparison of the expression of Ube1 in different developmental stages of testis and Sertoli cells (real-time PCR) indicated that Ube1 was expressed more highly in spermatogonia than in spermatocytes, spermatids and Sertoli cells. In conclusion, Ube1 is a gene encoding rat ubiquitin-activating enzyme E1 and specifically expressed in testis, which might play a key role in ubiquitin system and influence spermatogenesis.
通过抑制性消减杂交（SSH）和cDNA末端快速扩增（RACE）技术，利用大鼠A型精原细胞和粗线期精母细胞的总RNA，鉴定并表征了一个可能参与精子发生的基因。该基因由3433个碱基对（bp）组成，具有一个3171 bp的完整开放阅读框（ORF），编码一个含有1057个氨基酸的推定蛋白。核苷酸序列与小鼠泛素激活酶E1、Y染色体1（Ube1y1）具有93%的同一性，与人类泛素激活酶E1（UBE1）具有82%的同一性。该基因的推定蛋白包含一个泛素激活酶特征1和一个泛素激活酶活性位点，这些也存在于小鼠泛素激活酶E1、人类泛素激活酶E1等中。因此，我们将该基因命名为褐家鼠泛素激活酶E1（Ube1）。Ube1的序列已提交至GenBank，登录号为EF690356。逆转录-聚合酶链反应（RT-PCR）分析表明，Ube1在睾丸中特异性表达，而在心脏、大脑、脾脏、肺、肝脏、肌肉、肾脏和卵巢中未检测到其表达。睾丸不同发育阶段和支持细胞中Ube1表达的比较（实时PCR）表明，Ube1在精原细胞中的表达高于精母细胞、精子细胞和支持细胞。总之，Ube1是一个编码大鼠泛素激活酶E1的基因，在睾丸中特异性表达，可能在泛素系统中起关键作用并影响精子发生。
