Larson T G, Goodgal S H
Graduate Group in Biochemistry, University of Pennsylvania, School of Medicine, Philadelphia 19104.
J Bacteriol. 1991 Aug;173(15):4683-91. doi: 10.1128/jb.173.15.4683-4691.1991.
A 2.8-kb EcoRI-BglII fragment cloned from the wild-type Haemophilus influenzae Rd chromosome is shown to increase the transformability of the Com-101 mutant through trans complementation. Deletion and sequence analyses indicate that the active region of the clone carries a 687-bp open reading frame. A 0.3-kb insertion in the corresponding EcoRI-BglII fragment of the Com-101 chromosome is shown to be a partial (331-bp) duplication of this open reading frame. The wild-type sequence produces a peptide of a size that is consistent with the sequence data when this sequence is expressed in Escherichia coli with a T7 promoter-based transcription vector. RNA hybridization analysis using a DNA probe derived from the open reading frame suggests that the sequence is transiently expressed during competence development. On the basis of these observations, it is proposed that the open reading frame corresponds to the com101A gene.
从野生型流感嗜血杆菌Rd染色体克隆的一个2.8kb的EcoRI - BglII片段，通过反式互补作用可提高Com - 101突变体的转化能力。缺失和序列分析表明，该克隆的活性区域带有一个687bp的开放阅读框。Com - 101染色体相应的EcoRI - BglII片段中有一个0.3kb的插入片段，被证明是这个开放阅读框的部分（331bp）重复。当该序列在带有基于T7启动子的转录载体的大肠杆菌中表达时,野生型序列产生的一种肽的大小与序列数据一致。使用来自开放阅读框的DNA探针进行RNA杂交分析表明，该序列在感受态发育过程中短暂表达。基于这些观察结果，推测该开放阅读框对应于com101A基因。
