Herriott R M, Meyer E M, Vogt M
J Bacteriol. 1970 Feb;101(2):517-24. doi: 10.1128/jb.101.2.517-524.1970.
The composition of a defined nongrowth medium used in stage II development of competence of Haemophilus influenzae affects the course of this development. The development of competence in two nongrowth media, M-IV and M-V, is rapid, logarithmic, and independent of the cell concentration. This last property indicates that there is probably no transfer of a competence factor from competent to noncompetent cells, in contrast to results reported for other organisms. Levels of competence reached in these completely defined media are such that 1 to 5% of the cells are transformed in the presence of an excess of marked deoxyribonucleic acid. The method of evaluating competence, which depends on the frequency of multiple independent transformations, has been reexamined. This and other methods are compared on samples taken from a culture during development of competence.
用于流感嗜血杆菌感受态II期发育的特定非生长培养基的组成会影响这一发育过程。在两种非生长培养基M-IV和M-V中，感受态的发育迅速、呈对数增长，且与细胞浓度无关。这一特性表明，与其他生物体的报道结果相反，感受态细胞可能不会向非感受态细胞传递感受态因子。在这些完全确定的培养基中达到的感受态水平使得在存在过量标记脱氧核糖核酸的情况下，1%至5%的细胞会发生转化。对依赖多次独立转化频率来评估感受态的方法进行了重新审视。将该方法及其他方法用于在感受态发育过程中从培养物中采集的样本并进行比较。
