Chandler M S
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1626-30. doi: 10.1073/pnas.89.5.1626.
The Haemophilus influenzae Rd strain JG87 contains a single mini-Tn10kan insertion that causes a deficiency in the development of competence for genetic transformation. The DNA fragment containing this insertion mutation, as well as the wild-type locus, was cloned, mapped, and sequenced. The sequence contained an open reading frame for a protein of 224 amino acids with a predicted Mr of 25,152. The deduced protein sequence showed strong similarity to the Escherichia coli cAMP receptor protein. The E. coli crp gene cloned on a multicopy plasmid was shown to fully complement the competence-deficient phenotype of the mutant strain; thus, the H. influenzae gene was named crp. These results suggest that H. influenzae cAMP-cAMP receptor protein complex functions to regulate one or more promoters essential for the development of competence in H. influenzae Rd. Features of a gene upstream of H. influenzae crp that is homologous to the E. coli ttk gene are also described.
流感嗜血杆菌Rd菌株JG87含有一个单一的mini-Tn10kan插入片段，该片段导致遗传转化能力发育缺陷。包含此插入突变的DNA片段以及野生型基因座被克隆、定位并测序。该序列包含一个224个氨基酸的蛋白质的开放阅读框，预测分子量为25,152。推导的蛋白质序列与大肠杆菌cAMP受体蛋白有很强的相似性。克隆在多拷贝质粒上的大肠杆菌crp基因被证明能完全互补突变菌株的能力缺陷表型；因此，流感嗜血杆菌基因被命名为crp。这些结果表明，流感嗜血杆菌cAMP-cAMP受体蛋白复合物发挥功能，调节流感嗜血杆菌Rd中能力发育所必需的一个或多个启动子。还描述了流感嗜血杆菌crp上游一个与大肠杆菌ttk基因同源的基因的特征。
