大鼠胰岛素原前体mRNA的部分纯化及特性分析
Partial purification and characterization of the mRNA for rat preproinsulin.
作者信息
Duguid J R, Steiner D F, Chick W L
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3539-43. doi: 10.1073/pnas.73.10.3539.
Abstract
Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.
摘要
通过使用寡聚（dT）-纤维素层析和蔗糖密度梯度离心法，从胰岛细胞瘤中制备了大鼠胰岛素原前体的电泳均一信使核糖核酸（mRNA）。通过在甲酰胺中进行聚丙烯酰胺凝胶电泳测定，该mRNA的分子量约为210,000，在含有0.2M氯化钠的蔗糖梯度中其沉降系数为9.3S。这些结果表明，胰岛素原前体的合成由长度为600个核苷酸的单顺反子mRNA指导，并且需要该分子约55%的编码能力。
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