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Construction and selection of recombinant plasmids containing full-length complementary DNAs corresponding to rat insulins I and II.

作者信息

Chan S J, Noyes B E, Agarwal K L, Steiner D F

出版信息

Proc Natl Acad Sci U S A. 1979 Oct;76(10):5036-40. doi: 10.1073/pnas.76.10.5036.

DOI:10.1073/pnas.76.10.5036
PMID:388427
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC413074/
Abstract

We have used a synthetic deoxydecanucleotide to generate an insulin-specific cDNA probe suitable for selecting transformants that contain nearly full-length cDNAs corresponding to the mRNAs coding for rat insulins I and II. Double-stranded cDNA was synthesized from x-ray-induced rat insulinoma poly(A)-RNA, inserted in pBR322 plasmid DNA by the homopolymeric tailing technique, and cloned in Escherichia coli chi 1776. Colony hybridization with oligonucleotide-primed cDNA yielded 16 positive clones of which 7 corresponded to rat insulin I mRNA and 9 to rat insulin II mRNA. Restriction endonuclease maps of representative clones of each group indicated that these contained the complete coding sequences, as was confirmed by nucleotide sequence analysis of the 5' region of the cloned DNA for rat insulin II. Nucleotide sequence analysis also established the amino acid sequence of the prepeptide of rat preproinsulin II. Comparison of the amino acid sequence of the prepeptides of rat preproinsulin I and II shows that three conservative amino acid substitutions have occurred in this region of the molecule.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/92579174b98e/pnas00010-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/14b3f040f3f1/pnas00010-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/16747b798ff7/pnas00010-0297-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/92579174b98e/pnas00010-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/14b3f040f3f1/pnas00010-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/16747b798ff7/pnas00010-0297-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdf0/413074/92579174b98e/pnas00010-0298-a.jpg

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本文引用的文献

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DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
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Species variation in the amino acid sequence of insulin.胰岛素氨基酸序列中的物种差异。
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Insulin biosynthesis in the rat: demonstration of two proinsulins.大鼠体内的胰岛素生物合成：两种胰岛素原的证明。
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新生期链脲佐菌素诱导糖尿病大鼠中葡萄糖对正常β细胞调节的不可逆丧失。
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Nuclear translocation of a maternal CCAAT factor at the start of gastrulation activates Xenopus GATA-2 transcription.原肠胚形成开始时母体CCAAT因子的核转位激活了非洲爪蟾GATA-2转录。
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Cytokines suppress human islet function irrespective of their effects on nitric oxide generation.细胞因子抑制人类胰岛功能，无论其对一氧化氮生成的影响如何。
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The application of DNA recombinant technology to the analysis of the human genome and genetic disease.DNA重组技术在人类基因组和遗传疾病分析中的应用。
Hum Genet. 1981;58(4):351-7. doi: 10.1007/BF00282814.
7
Molecular cloning and nucleotide sequence of full-length of cDNA coding for porcine gastrin.猪胃泌素编码全长cDNA的分子克隆及核苷酸序列
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1049-53. doi: 10.1073/pnas.79.4.1049.
8
Recent developments in the chemical synthesis of polynucleotides.多核苷酸化学合成的最新进展。
Nucleic Acids Res. 1982 Nov 11;10(21):6553-70. doi: 10.1093/nar/10.21.6553.
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Proc Natl Acad Sci U S A. 1984 Jun;81(12):3635-9. doi: 10.1073/pnas.81.12.3635.
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