卵清蛋白信使核糖核酸：转录初始产物与多核糖体卵清蛋白信使大小相同的证据。
Ovalbumin messenger RNA: evidence that the initial product of transcription is the same size as polysomal ovalbumin messenger.
作者信息
McKnight G S, Schimke R T
出版信息
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4327-31. doi: 10.1073/pnas.71.11.4327.
Abstract
The messenger RNA for ovalbumin, the major secretory protein of the chick oviduct, appears not to be made as a high-molecular-weight precursor when artifacts due to aggregation are eliminated. No ovalbumin messenger RNA sequences that will hybridize to complementary DNA made against ovalbumin mRNA are found in concentrated samples of hen oviduct RNA larger than 28 S. The sensitivity of the hybridization assay is sufficient to detect less than one molecule of ovalbumin mRNA precursor per tubular gland cell. Newly synthesized ovalbumin messenger RNA isolated from immature chicks stimulated briefly by estrogen is the same size as that found in hen polyribosomes. We conclude that ovalbumin messenger RNA does not undergo any significant change in molecular weight from its initial transcription to its incorporation into polyribosomes.
摘要
卵清蛋白是鸡输卵管的主要分泌蛋白，当消除聚集造成的假象后，其信使核糖核酸似乎并非以高分子量前体的形式合成。在大于28 S的母鸡输卵管RNA浓缩样品中，未发现能与针对卵清蛋白信使核糖核酸制备的互补脱氧核糖核酸杂交的卵清蛋白信使核糖核酸序列。杂交检测的灵敏度足以检测到每个管状腺细胞中少于一个分子的卵清蛋白信使核糖核酸前体。从未成熟小鸡中分离出的、经雌激素短暂刺激后新合成的卵清蛋白信使核糖核酸，其大小与母鸡多核糖体中的相同。我们得出结论，卵清蛋白信使核糖核酸从最初转录到掺入多核糖体的过程中，分子量没有发生任何显著变化。
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