Lou Ping, Zhao Jianjun, He Hongju, Hanhart Corrie, Pino Del Carpio Dunia, Verkerk Ruud, Custers Jan, Koornneef Maarten, Bonnema Guusje
Laboratory of Plant Breeding, Wageningen University, 6700AJ, Wageningen, the Netherlands.
Horticultural College, Hebei Agricultural University, 071001, Baoding, China.
New Phytol. 2008;179(4):1017-1032. doi: 10.1111/j.1469-8137.2008.02530.x. Epub 2008 Jun 28.
Glucosinolates and their breakdown products have been recognized for their effects on plant defense, human health, flavor and taste of cruciferous vegetables. Despite this importance, little is known about the regulation of the biosynthesis and degradation in Brassica rapa. Here, the identification of quantitative trait loci (QTL) for glucosinolate accumulation in B. rapa leaves in two novel segregating double haploid (DH) populations is reported: DH38, derived from a cross between yellow sarson R500 and pak choi variety HK Naibaicai; and DH30, from a cross between yellow sarson R500 and Kairyou Hakata, a Japanese vegetable turnip variety. An integrated map of 1068 cM with 10 linkage groups, assigned to the international agreed nomenclature, is developed based on the two individual DH maps with the common parent using amplified fragment length polymorphism (AFLP) and single sequence repeat (SSR) markers. Eight different glucosinolate compounds were detected in parents and F(1)s of the DH populations and found to segregate quantitatively in the DH populations. QTL analysis identified 16 loci controlling aliphatic glucosinolate accumulation, three loci controlling total indolic glucosinolate concentration and three loci regulating aromatic glucosinolate concentrations. Both comparative genomic analyses based on Arabidopsis-Brassica rapa synteny and mapping of candidate orthologous genes in B. rapa allowed the selection of genes involved in the glucosinolate biosynthesis pathway that may account for the identified QTL.
硫代葡萄糖苷及其分解产物因其对植物防御、人类健康、十字花科蔬菜风味和口感的影响而受到认可。尽管其具有重要性，但关于芜菁中硫代葡萄糖苷生物合成和降解的调控却知之甚少。在此，报道了在两个新的分离双单倍体（DH）群体中芜菁叶片硫代葡萄糖苷积累的数量性状位点（QTL）鉴定：DH38群体源自黄芥R500与小白菜品种HK Naibaicai的杂交；DH30群体源自黄芥R500与日本蔬菜芜菁品种Kairyou Hakata的杂交。基于使用扩增片段长度多态性（AFLP）和单序列重复（SSR）标记的具有共同亲本的两个独立DH图谱，构建了一张1068 cM、包含10个连锁群的综合图谱，并赋予其国际认可的命名法。在DH群体的亲本和F1代中检测到8种不同的硫代葡萄糖苷化合物，且发现它们在DH群体中呈数量性状分离。QTL分析鉴定出16个控制脂肪族硫代葡萄糖苷积累的位点、3个控制总吲哚族硫代葡萄糖苷浓度的位点以及3个调节芳香族硫代葡萄糖苷浓度的位点。基于拟南芥 - 芜菁共线性的比较基因组分析以及芜菁中候选直系同源基因的定位，均有助于选择参与硫代葡萄糖苷生物合成途径的基因，这些基因可能解释所鉴定出的QTL。
