Li Zhansheng, Liu Guangmin, He Hongju, Liu Yumei, Han Fengqing, Liu Wei
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, and Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Institute of Agri-food Processing and Nutrition, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China.
Food Chem X. 2022 Aug 12;15:100429. doi: 10.1016/j.fochx.2022.100429. eCollection 2022 Oct 30.
The effects of a nanocarbon solution on the nutrients, glucosinolate metabolism and glucoraphanin pathway in broccoli were investigated. Significant positive linear relationships were observed between the nanocarbon solution and total protein yield, although effects on the soluble sugars, vitamin C and dry matter production were not observed. All nanocarbon solutions significantly increased the glucoraphanin content ( < 0.05), and the 18.75 L·ha nanocarbon solution maximally increased the glucoraphanin content by 22.9 %. However, these treatments also significantly reduced the contents of glucobrassicin, 4-methoxyglucobrassicin, 4-hydroxyglucobrassicin and neoglucobrassicin. Further research demonstrated that the 18.75 L·ha nanocarbon solution significantly upregulated the , , , , , and expression levels, which directly resulted in the accumulation of glucoraphanin and glucoerucin. This study provides insights into the prospective nanotechnological approaches for developing efficient and environmentally friendly nanocarbon solution for use on crops.
研究了纳米碳溶液对西兰花营养成分、硫代葡萄糖苷代谢及萝卜硫苷途径的影响。纳米碳溶液与总蛋白产量之间存在显著的正线性关系，不过未观察到其对可溶性糖、维生素C和干物质产量的影响。所有纳米碳溶液均显著提高了萝卜硫苷含量（P<0.05），其中18.75 L·ha的纳米碳溶液使萝卜硫苷含量最大增幅达22.9%。然而，这些处理也显著降低了葡萄糖异硫氰酸酯、4-甲氧基葡萄糖异硫氰酸酯、4-羟基葡萄糖异硫氰酸酯和新葡萄糖异硫氰酸酯的含量。进一步研究表明，18.75 L·ha的纳米碳溶液显著上调了[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达水平，这直接导致了萝卜硫苷和葡萄糖芥苷的积累。本研究为开发用于作物的高效且环保的纳米碳溶液的前瞻性纳米技术方法提供了见解。
