组织中药物和代谢物的基质辅助激光解吸电离傅里叶变换离子回旋共振成像质谱分析
MALDI-FTICR imaging mass spectrometry of drugs and metabolites in tissue.
作者信息
Cornett Dale S, Frappier Sara L, Caprioli Richard M
机构信息
Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.
出版信息
Anal Chem. 2008 Jul 15;80(14):5648-53. doi: 10.1021/ac800617s. Epub 2008 Jun 20.
Abstract
A new approach is described for imaging mass spectrometry analysis of drugs and metabolites in tissue using matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR). The technique utilizes the high resolving power to produce images from thousands of ions measured during a single mass spectrometry (MS)-mode experiment. Accurate mass measurement provides molecular specificity for the ion images on the basis of elemental composition. Final structural confirmation of the targeted compound is made from accurate mass fragment ions generated in an external quadrupole-collision cell. The ability to image many small molecules in a single measurement with high specificity is a significant improvement over existing MS/MS based technologies. Example images are shown for olanzapine in kidney and liver and imatinib in glioma.
摘要
本文描述了一种使用基质辅助激光解吸电离-傅里叶变换离子回旋共振（MALDI-FTICR）对组织中的药物和代谢物进行成像质谱分析的新方法。该技术利用高分辨率在单次质谱（MS）模式实验中测量的数千个离子生成图像。精确质量测量基于元素组成，为离子图像提供分子特异性。目标化合物的最终结构确认由外部四极杆碰撞池中产生的精确质量碎片离子完成。在单次测量中以高特异性对许多小分子进行成像的能力是对现有基于MS/MS的技术的重大改进。展示了肾脏和肝脏中奥氮平以及胶质瘤中伊马替尼的示例图像。
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