García-Rojas Nancy Shyrley, Sierra-Álvarez Carlos Daniel, Ramos-Aboites Hilda E, Moreno-Pedraza Abigail, Winkler Robert
Unidad de Genómica Avanzada, Cinvestav, Km. 9.6 Libramiento Norte Carr. Irapuato-León, Irapuato 36824, Mexico.
Leibniz Institute of Vegetable and Ornamental Crops (IGZ) e.V., Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.
Metabolites. 2024 Jul 30;14(8):419. doi: 10.3390/metabo14080419.
The presence and localization of plant metabolites are indicative of physiological processes, e.g., under biotic and abiotic stress conditions. Further, the chemical composition of plant parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique for exploring and visualizing the spatial distribution of plant molecules within a tissue. This review provides a summary of mass spectrometry methods used for mapping and identifying metabolites in plant tissues. We present the benefits and the disadvantages of both vacuum and ambient ionization methods, considering direct and indirect approaches. Finally, we discuss the current limitations in annotating and identifying molecules and perspectives for future investigations.
植物代谢物的存在和定位表明了生理过程,例如在生物和非生物胁迫条件下。此外,植物各部分的化学成分与其作为食物或药用的质量相关。质谱成像(MSI)已成为一种流行的分析技术,用于探索和可视化植物分子在组织内的空间分布。本综述总结了用于绘制和识别植物组织中代谢物的质谱方法。我们考虑直接和间接方法,介绍了真空和常压电离方法的优缺点。最后,我们讨论了目前在分子注释和识别方面的局限性以及未来研究的前景。