Tsai Cheng-Chih, Lai Chieh-Hsien, Yu Bi, Tsen Hau-Yang
Department of Food Science and Nutrition, Hung-Kuang University, No. 34, Chung-Chi Road, Shalu, Taichung County 433, Taiwan, ROC.
Anaerobe. 2008 Oct;14(4):219-23. doi: 10.1016/j.anaerobe.2008.05.001. Epub 2008 May 17.
Effective methods for the identification and enumeration of lactic acid producing bacteria (LAB) cells are important for the quality control and assurance of probiotic products. In this study, we designed a polymerase chain reaction (PCR) primer set from the sequence in 16S-23S internal transcribed spacer (ITS) region and used it for the specific detection of Bifidobacterium adolescentis, one of the Bifidobacterium species used in probiotics. Specificity of the PCR primers, i.e., bits-1/bits-2, was assured by assay strains of B. adolescentis, other Bifidobacterium species, and strains of non-Bifidobacterium spp. Coupled with the use of a known primer set specific for Bifidobacterium species, Bifidobacterium strains and B. adolescentis could be identified from LAB strains in fermented dairy products and human fecal samples.
用于鉴定和计数产乳酸菌（LAB）细胞的有效方法对于益生菌产品的质量控制和保证至关重要。在本研究中，我们根据16S - 23S内转录间隔区（ITS）区域的序列设计了一组聚合酶链反应（PCR）引物，并将其用于特异性检测青春双歧杆菌，这是一种用于益生菌的双歧杆菌属物种。通过青春双歧杆菌、其他双歧杆菌属物种的测定菌株以及非双歧杆菌属物种的菌株来确保PCR引物（即bits - 1/bits - 2）的特异性。结合使用一组已知的针对双歧杆菌属物种的特异性引物，可以从发酵乳制品和人类粪便样本中的LAB菌株中鉴定出双歧杆菌菌株和青春双歧杆菌。
