Yang Xue-Lei, Matthijnssens Jelle, Sun He, Muhamaiti Jianaer, Zhang Bin, Nahar Shamsun, Van Ranst Marc, Rahman Mustafizur
Xinjiang Pediatric Institute, People's Hospital of Xinjiang, Urumqi, People's Republic of China.
Int J Infect Dis. 2008 Nov;12(6):e11-7. doi: 10.1016/j.ijid.2008.03.022. Epub 2008 Jun 18.
Very little is known about human rotaviruses in the northwest of China. To investigate the genomic diversity, we evaluated the distribution of rotavirus genotypes in this region covering a 10-year period (1996-2005).
Rotavirus antigen was detected in stool specimens by enzyme immunoassay (EIA), and G and P genotyping was performed by reverse transcription-polymerase chain reaction and nucleotide sequencing methods.
A total of 783 stool specimens collected from children with diarrhea, under 5 years of age, attending an urban hospital in Xinjiang were tested for rotavirus antigen, and 398 (50.8%) were positive. Overall, the most prevalent rotavirus genotype was G1P[8] (40.0%), followed by G3P[8] (17.5%), G2P[4] (8.3%), and G2P[6] (6.5%). G1 rotavirus was the most prevalent genotype until 2004. However, in 2005, G3 rotavirus (51.9%) became a dominating strain. Only one G9 strain was isolated in this region (isolated for the first time in 1999) and it became a more prevalent strain (21.2%) in 2005.
The results of this study are of importance to the decision makers in the evaluation of rotavirus vaccines in China.
关于中国西北部地区的人类轮状病毒，人们了解甚少。为了研究基因组多样性，我们评估了该地区10年期间（1996 - 2005年）轮状病毒基因型的分布情况。
采用酶免疫分析法（EIA）检测粪便标本中的轮状病毒抗原，并通过逆转录 - 聚合酶链反应和核苷酸测序方法进行G和P基因型分型。
对来自新疆一家城市医院的783份5岁以下腹泻儿童的粪便标本进行了轮状病毒抗原检测，其中398份（50.8%）呈阳性。总体而言，最常见的轮状病毒基因型是G1P[8]（40.0%），其次是G3P[8]（17.5%）、G2P[4]（8.3%）和G2P[6]（6.5%）。直到2004年，G1轮状病毒一直是最常见的基因型。然而，在2005年，G3轮状病毒（51.9%）成为主导毒株。该地区仅分离出一株G9毒株（于1999年首次分离），并在2005年成为更常见的毒株（21.2%）。
本研究结果对中国轮状病毒疫苗评估的决策者具有重要意义。
