Meulewaeter F, Seurinck J, Van Emmelo J
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Virology. 1990 Aug;177(2):699-709. doi: 10.1016/0042-6822(90)90536-z.
An almost complete sequence of the RNA genome of tobacco necrosis virus (TNV) strain A has been determined. The genome organization is very similar to that of carnation mottle virus (CarMV) and turnip crinkle virus (TCV). The 5'-proximal open reading frame (ORF) encodes a 23-kDa protein and read-through of its amber codon into the second ORF is presumably used for the translation of a 82-kDa protein. The third large ORF encodes the 30-kDa coat protein. Two small ORFs are located upstream and one immediately downstream of this coat protein cistron. Extensive sequence similarity was found between the TNV 82-kDa protein and the putative polymerases of TCV, CarMV, cucumber necrosis virus (CNV), maize chlorotic mottle virus (MCMV), red clover necrotic mosaic virus (RCNMV), and barley yellow dwarf virus (BYDV). The TNV coat protein is very similar to southern bean mosaic virus (SBMV) capsid protein. Of the predicted small proteins only a 7.9-kDa protein shows some sequence similarity with a corresponding protein of MCMV, CarMV, and TCV. The others are unique to TNV. Except for the first four nucleotides at the 5' end no homology was found with the RNA of STNV (satellite of TNV).
烟草坏死病毒A株（TNV）RNA基因组的几乎完整序列已被测定。其基因组结构与香石竹斑驳病毒（CarMV）和芜菁皱缩病毒（TCV）非常相似。5'-近端开放阅读框（ORF）编码一种23 kDa的蛋白质，其琥珀密码子通读至第二个ORF，推测用于翻译一种82 kDa的蛋白质。第三个大ORF编码30 kDa的外壳蛋白。在该外壳蛋白顺反子的上游和紧邻下游分别有两个小ORF。在TNV的82 kDa蛋白与TCV、CarMV、黄瓜坏死病毒（CNV）、玉米褪绿斑驳病毒（MCMV）、红三叶草坏死花叶病毒（RCNMV）以及大麦黄矮病毒（BYDV）的假定聚合酶之间发现了广泛的序列相似性。TNV外壳蛋白与南方菜豆花叶病毒（SBMV）的衣壳蛋白非常相似。在预测的小蛋白中，只有一种7.9 kDa的蛋白与MCMV、CarMV和TCV相应蛋白表现出一定序列相似性。其他小蛋白是TNV所特有的。除了5'端的前四个核苷酸外，未发现与STNV（TNV的卫星病毒）的RNA有同源性。
