Grieco F, Savino V, Martelli G P
Dipartimento di Protezione delle Piante, Università degli Studi, Bari, Italy.
Arch Virol. 1996;141(5):825-38. doi: 10.1007/BF01718158.
The 3699 nt genome of olive latent virus 1 (OLV-1), described years ago from Southern Italy as a putative sobemovirus, was completely sequenced. OLV-1 genomic RNA was not polyadenylated and had a structure virtually identical to that of species of the Necrovirus rather than the Sobemovirus genus. Five open reading frames (ORFs) were identified, of which the 5'-proximal encoded a 23K protein and ended with an amber codon whose readthrough could yield a putative 82K product. This polypeptide had extensive sequence similarity with polymerases of serotypes A and D of tobacco necrosis necrovirus (TNV-A and TNV-D) and species of the family Tombusviridae and related genera (Dianthovirus and Machlomovirus). Two small ORFs followed, which encoded polypeptides of 8K and 6K, respectively. The 6K product had extensive homology with the comparable 6K protein of TNV-A and was also related to the 11K protein of shallot latent carlavirus, one of the "triple block" polypeptides involved in cell-to-cell virus movement. The 3'-proximal ORF was in the same position as the coat protein (CP) cistron of necroviruses and encoded a 30K product related to CP of both TNV-A and -D. Computer-assisted comparative analysis of structural and non-structural proteins of OLV-1, TNV-A and TNV-D disclosed on overall distant relationship between OLV-1 and TNV-D. OLV-1 genome appeared homologous to that of TNV-A, but differences from TNV-A were the absence of the small ORF downstream of the CP cistron and in the low degree of sequence identity in CP (39% aa identity). OLV-1 is serologically distantly related to TNV-A and even more distantly related to TNV-D. We propose that OLV-1 is a necrovirus species in its own right.
多年前在意大利南部被描述为一种假定的南方菜豆花叶病毒的油橄榄潜隐病毒1（OLV-1）的3699个核苷酸的基因组已被完全测序。OLV-1基因组RNA没有聚腺苷酸化，其结构实际上与坏死病毒属的物种相同，而不是南方菜豆花叶病毒属。鉴定出了五个开放阅读框（ORF），其中5'-近端的开放阅读框编码一种23K蛋白，并以琥珀密码子结尾，其通读可产生一种假定的82K产物。该多肽与烟草坏死坏死病毒（TNV-A和TNV-D）的A和D血清型的聚合酶以及番茄丛矮病毒科及其相关属（石竹病毒属和麦拉病毒属）的物种具有广泛的序列相似性。随后是两个小的开放阅读框，分别编码8K和6K的多肽。6K产物与TNV-A的可比6K蛋白具有广泛的同源性，并且还与青葱潜隐香石竹病毒的11K蛋白相关，该蛋白是参与病毒细胞间移动的“三联体阻断”多肽之一。3'-近端的开放阅读框与坏死病毒的衣壳蛋白（CP）顺反子处于相同位置，并编码一种与TNV-A和-D的CP相关的30K产物。对OLV-1、TNV-A和TNV-D的结构和非结构蛋白进行的计算机辅助比较分析揭示了OLV-1与TNV-D之间的总体远缘关系。OLV-1基因组似乎与TNV-A的基因组同源，但与TNV-A的不同之处在于CP顺反子下游没有小的开放阅读框，并且CP中的序列同一性程度较低（氨基酸同一性为39%）。OLV-1在血清学上与TNV-A远缘相关，与TNV-D的关系更远。我们提出OLV-1本身就是一种坏死病毒物种。
