Bagger S, Breddam K, Byberg B R
Chemistry Department A, Technical University of Denmark, Lyngby.
J Inorg Biochem. 1991 May 1;42(2):97-103. doi: 10.1016/0162-0134(91)80036-h.
Chemically modified enzymes have been prepared by incorporating an -Hg-L group into proteinase K and carboxypeptidase Y at the thiol groups of Cys-73 and Cys-341, respectively (L = CN- or I-). The -S-Hg-13CN group has been applied as a spectroscopic label for carbon-13 NMR spectroscopy.
通过分别在蛋白酶K的Cys-73巯基和羧肽酶Y的Cys-341巯基处引入-Hg-L基团（L = CN-或I-）制备了化学修饰酶。-S-Hg-13CN基团已用作碳-13核磁共振光谱的光谱标记。
