Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Institute of Biological Study, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Low Dimensional Material Research Center (LDMRC), Department of Physics, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Int J Biol Macromol. 2019 Jul 1;132:615-628. doi: 10.1016/j.ijbiomac.2019.03.235. Epub 2019 Mar 30.
In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1-1.0 M), chitosan coupling pH (6-8), chitosan coupling concentration (0.1-3.0%), algal solution pH (6-8), algal mass concentration (0.1-1.0% dw/v), and membrane flux (4.08 × 10-2.04 × 10 mL/min·cm) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.
在这项工作中，制备了壳聚糖改性纳米纤维膜，并用于研究从螺旋藻中获得的藻蓝蛋白 (CPC) 的渗透特性。考察了 NaCl 浓度（0.1-1.0 M）、壳聚糖偶联 pH 值（6-8）、壳聚糖偶联浓度（0.1-3.0%）、藻液 pH 值（6-8）、藻液质量浓度（0.1-1.0% dw/v）和膜通量（4.08×10-2.04×10 mL/min·cm）对 CPC 穿透膜性能的影响。结果表明，该膜对这些蛋白质的结合选择性顺序为：污染蛋白（TP）>别藻蓝蛋白（APC）>CPC。TP 和 APC 分子更容易被壳聚糖改性膜吸附，而 CPC 分子最容易穿透膜而不被吸附，从而提高了 CPC 的纯度。在单一步骤中，纯化因子和总质量通量分别提高了 3.3 倍和 66%。
