Yu Zuoren, Hecht Norman B
Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, 1310 Biomedical Research Building, 421 Curie Blvd, Philadelphia, PA 19104-6080, USA.
J Androl. 2008 Sep-Oct;29(5):572-9. doi: 10.2164/jandrol.108.005090. Epub 2008 Jun 20.
Translin (TSN), also known as testis-brain RNA-binding protein, is proposed to bind to breakpoint junctions at chromosomal translocations in the nucleus and to specific RNAs in the cytoplasm. In germ cells of the mouse testis, it recognizes target mRNAs transcribed by the transcription factor CREM-tau in spermatids, specific meiotically expressed mRNAs, and a noncoding RNA that encodes piRNAs. Here we show that TSN also binds to the microRNA miR-122a. MiR-122a is expressed in late-stage germ cells and is complementary to a sequence in the 3' untranslated region of the transition protein 2 mRNA. The binding of TSN to miR-122a increases its in vivo stability, suggesting an additional posttranscriptional function for TSN.
易位蛋白（TSN），也被称为睾丸-脑RNA结合蛋白，被认为可在细胞核中与染色体易位处的断点连接点结合，并在细胞质中与特定RNA结合。在小鼠睾丸的生殖细胞中，它能识别由转录因子CREM-tau在精子细胞中转录的靶mRNA、特定减数分裂期表达的mRNA以及一种编码piRNA的非编码RNA。在此我们表明，TSN还能与微小RNA miR-122a结合。MiR-122a在晚期生殖细胞中表达，并且与过渡蛋白2 mRNA的3'非翻译区中的一个序列互补。TSN与miR-122a的结合增加了其体内稳定性，这表明TSN具有额外的转录后功能。
