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基于16S rRNA基因序列分析揭示粒径对水生沉积物细菌群落结构的影响

Influence of particle size on bacterial community structure in aquatic sediments as revealed by 16S rRNA gene sequence analysis.

作者信息

Jackson Colin R, Weeks Andrew Q

机构信息

Department of Biology, Shoemaker Hall, The University of Mississippi, University, MS 38677, USA.

出版信息

Appl Environ Microbiol. 2008 Aug;74(16):5237-40. doi: 10.1128/AEM.00923-08. Epub 2008 Jun 20.

Abstract

Bacterial communities associated with sediment particles were examined using PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. Particle size influenced community structure, with attached bacterial assemblages separating into 63- to 125-, 125- to 1,000-, and 1,000- to 2,000-microm fractions. Differences were particularly pronounced for the Verrucomicrobia-Planctomycetes, whose numbers were significantly reduced on coarser particles.

摘要

利用聚合酶链式反应-变性梯度凝胶电泳和16S rRNA基因测序技术对与沉积物颗粒相关的细菌群落进行了检测。颗粒大小影响群落结构,附着的细菌群落分为63至125微米、125至1000微米以及1000至2000微米的组分。疣微菌纲-浮霉菌纲的差异尤为显著,其数量在较粗颗粒上显著减少。

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