Kumar A, Jindal N, Shukla C L, Pal Yash, Ledoux D R, Rottinghaus G E
Department of Veterinary Epidemiology and Preventive Medicine, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, India.
Avian Dis. 2003 Apr-Jun;47(2):415-24. doi: 10.1637/0005-2086(2003)047[0415:EOOAOE]2.0.CO;2.
A study was conducted to evaluate the effects of ochratoxin A (OA) on Escherichia coli-challenged broiler chickens. Day-old broiler chicks were separated into two groups of 92 chicks each, with one group fed a control mash diet, and the other fed a mash diet containing 2 ppm OA. On day 14, each group was further separated into two groups, with one group inoculated with E. coli O78 (1 x 10(7) colony-forming units/0.5 ml), whereas the other group was not inoculated with E. coli. After E. coli inoculation on day 14, four birds from each group were euthanatized at 1, 2, 3, 5, 7, 10, 14, and 21 days postinoculation. Escherichia coli infection caused dullness, depression, huddling, and diarrhea. Mortality was 14.3% in chicks infected with E. coli but fed no OA. Mortality increased to 35.7% in chicks fed OA and infected with E. coli. Decreased body weight and reduced feed intake were observed in chicks fed OA, and the effects were more pronounced in chicks fed OA and infected with E. coli. Increased serum levels of aspartate aminotransferase, alanine aminotransferase, uric acid, and creatinine and decreased levels of total proteins, albumin, globulins, calcium, and phosphorus were observed in OA-fed birds. Escherichia coli infection did not cause significant alteration in any of the serum biochemical parameters. The presence of OA in poultry rations increased mortality and the severity of an E. coli infection.
开展了一项研究以评估赭曲霉毒素A（OA）对受大肠杆菌攻击的肉鸡的影响。一日龄肉鸡雏鸡被分成两组，每组92只，一组饲喂对照粉料日粮，另一组饲喂含2 ppm OA的粉料日粮。在第14天，每组进一步分成两组，一组接种大肠杆菌O78（1×10⁷菌落形成单位/0.5 ml），而另一组不接种大肠杆菌。在第14天接种大肠杆菌后，在接种后1、2、3、5、7、10、14和21天从每组中安乐死4只鸡。大肠杆菌感染导致肉鸡精神萎靡、抑郁、扎堆和腹泻。未饲喂OA但感染大肠杆菌的雏鸡死亡率为14.3%。饲喂OA且感染大肠杆菌的雏鸡死亡率增至35.7%。观察到饲喂OA的雏鸡体重下降和采食量减少，且在饲喂OA并感染大肠杆菌的雏鸡中这些影响更明显。在饲喂OA的鸡中观察到血清天冬氨酸转氨酶、丙氨酸转氨酶、尿酸和肌酐水平升高，总蛋白、白蛋白、球蛋白、钙和磷水平降低。大肠杆菌感染未导致任何血清生化参数发生显著变化。家禽日粮中存在OA会增加死亡率以及大肠杆菌感染的严重程度。
