Antibacterial and preliminary phytochemical and physico-chemical analysis of Eucalyptus citriodora Hk leaf.

The present communication deals with some studies on the antibacterial, physico-chemical and phytochemical parameters of different extracts of Eucalyptus citriodora leaf. The antibacterial study was performed using the agar ditch method on some clinically important bacteria, namely Pseudomonas pseudoalcaligenes, Proteus vulgaris, Citrobacter freundii, Staphylococcus subflava, Bacillus megaterium, and Enterobacter aerogenes. Physico-chemical parameters namely water, methanol, 1,4-dioxane, DMF, acetone soluble extractives, total ash, melting point, and pH were determined according to pharmacopoeial procedures. Methanol gave the maximum extract while it was minimum in water. Phytochemical parameters were screened for alkaloids, tannins, cardiac glycosides, saponins, steroids and flavonoids. Tannins and flavonoids gave positive results, while steroids and glycosides were absent. The most susceptible bacteria was C. freundii, while the most resistant was P. vulgaris.