Luo Ke-Li, Fan Li-Qing, Lu Guang-Xiu
Research Institute of Reproduction and ES Cells Engineering, Xiangya Medical School, Central South University, Changsha, Hunan 410078, China.
Zhonghua Nan Ke Xue. 2008 May;14(5):431-5.
To compare the proteomic differences between normal and necrozoospermic human spermatozoa.
We isolated proteins from 30 ejaculates of 10 healthy donors and 3 specimens of a necrozoospermia patient by two-dimensional gel electrophoresis (2-DE), analyzed their 2-DE maps and identified some differentially expressed proteins by mass spectrometry.
A total of (905 +/- 57) and (792 +/- 28) proteins were isolated from the spermatozoal maps of the normal and necrozoospermic men, respectively, and 178 found to be differentially expressed in that of the necrozoospermia patient. The 6 missing protein spots in the necrozoospermic map were identified as sperm protein (human, accession No. 060904), zinc finger protein 174 (AW-1), F-actin capping protein alpha-3 subunit, testis-specific inhibitor of apoptosis, death domain receptor 3 soluble form (fragment) and peptide similar to the activator of CREM in the testis.
Six missing protein spots were identified in the necrozoospermic spermatozoal map which may be associated with the development of necrozoospermia.
比较正常人类精子与死精症患者精子的蛋白质组差异。
我们通过二维凝胶电泳（2-DE）从10名健康供体的30份精液以及1例死精症患者的3份样本中分离蛋白质，分析其二维电泳图谱，并通过质谱鉴定一些差异表达的蛋白质。
分别从正常男性和死精症男性的精子图谱中分离出（905±57）和（792±28）种蛋白质，发现其中178种在死精症患者中差异表达。死精症图谱中缺失的6个蛋白质点被鉴定为精子蛋白（人类，登录号060904）、锌指蛋白174（AW-1）、F-肌动蛋白封端蛋白α-3亚基、睾丸特异性凋亡抑制因子、死亡结构域受体3可溶性形式（片段）以及与睾丸中CREM激活剂相似的肽。
在死精症精子图谱中鉴定出6个缺失的蛋白质点，它们可能与死精症的发生有关。
