Institut National de la Santé et de la Recherche Médicale, Unité mixte de recherche 977, 11 rue Humann, 67085 Strasbourg Cédex, France.
Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 Place de l'Hôpital, 67000 Strasbourg, France.
Int J Mol Sci. 2009 Jul 28;10(8):3283-3315. doi: 10.3390/ijms10083283.
Isothermal titration microcalorimetry (ITC) is mostly used to investigate the thermodynamics of "specific" host-guest interactions in biology as well as in supramolecular chemistry. The aim of this review is to demonstrate that ITC can also provide useful information about non-specific interactions, like electrostatic or hydrophobic interactions. More attention will be given in the use of ITC to investigate polyelectrolyte-polyelectrolyte (in particular DNA-polycation), polyelectrolyte-protein as well as protein-lipid interactions. We will emphasize that in most cases these "non specific" interactions, as their definition will indicate, are favoured or even driven by an increase in the entropy of the system. The origin of this entropy increase will be discussed for some particular systems. We will also show that in many cases entropy-enthalpy compensation phenomena occur.
等温滴定量热法（ITC）主要用于研究生物学和超分子化学中“特定”主客体相互作用的热力学。本文的目的是证明 ITC 还可以提供有关非特异性相互作用（如静电相互作用或疏水相互作用）的有用信息。本文将更关注 ITC 在研究聚电解质-聚电解质（特别是 DNA-聚阳离子）、聚电解质-蛋白质以及蛋白质-脂质相互作用中的应用。我们将强调，在大多数情况下，这些“非特异性”相互作用（如其定义所示）是由系统熵的增加所促进甚至驱动的。对于一些特定的体系，我们将讨论这种熵增加的来源。我们还将表明，在许多情况下，熵-焓补偿现象会发生。
