Wang Bo, Sheng Jian-Zhong, He Rong-Huan, Qian Yu-Li, Jin Fan, Huang He-Feng
Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, 2 Xue Shi Road, Hangzhou, Zhejiang, China.
Am J Reprod Immunol. 2008 Aug;60(2):127-34. doi: 10.1111/j.1600-0897.2008.00604.x.
L-selectin ligand has displayed mediating adhesion at the maternal-fetal interface. Therefore, we investigated the impact of L-selectin ligand on establishing pregnancy in women undergoing in vitro fertilization and embryo transfer (IVF-ET).
Endometrium between cycle days LH +6 to +9 was obtained from 56 Chinese women referred for IVF and tested for L-selectin ligand by immunohistochemistry and Western blot. The standard gonadotropin-releasing hormone agonist long protocol was used for ovarian stimulation.
L-selectin ligand was localized in the endometrial gland and luminal epithelial cells. Western blot analysis of endometrium identified four bands and levels of component 1, 2 and 4 were significantly higher in the pregnancy group than in the non-pregnancy group (P < 0.05). Clinical pregnancy and implantation rates were higher in patients with high level L-selectin ligand compared with those with low level (53.6%versus 25.0%, and 27.1%versus 12.1%, respectively, P < 0.05).
The presence of higher level L-selectin ligand was associated with a better pregnancy outcome.
L-选择素配体已显示出在母胎界面介导黏附的作用。因此，我们研究了L-选择素配体对接受体外受精和胚胎移植（IVF-ET）的女性建立妊娠的影响。
从56名因IVF前来就诊的中国女性中获取月经周期中LH +6至+9天之间的子宫内膜，通过免疫组织化学和蛋白质印迹法检测L-选择素配体。采用标准的促性腺激素释放激素激动剂长方案进行卵巢刺激。
L-选择素配体定位于子宫内膜腺体和腔上皮细胞中。子宫内膜的蛋白质印迹分析鉴定出四条条带，妊娠组中成分1、2和4的水平显著高于非妊娠组（P < 0.05）。L-选择素配体水平高的患者临床妊娠率和着床率高于水平低的患者（分别为53.6%对25.0%，27.1%对12.1%，P < 0.05）。
较高水平的L-选择素配体的存在与更好的妊娠结局相关。
