Ciprandi Giorgio, Continia Paola, Fenoglio Daniela, Sormani Maria Pia, Negrini Simone, Puppo Francesco, Indiveri Francesco
Department of Internal Medicine, University of Genoa, Genoa, Italy.
Hum Immunol. 2008 Jul;69(7):409-13. doi: 10.1016/j.humimm.2008.05.009. Epub 2008 Jun 23.
Allergic rhinitis (AR) is characterized by a T-helper (Th)-2 (Th2) polarized immune response. Soluble human leukocyte antigen (sHLA) molecules play an immunomodulatory role. Specific immunotherapy is the only causal treatment for AR and is able to shift the immune response to Th1 polarization. The aim of this study was to evaluate the relationship between sHLA-G and sHLA-A,-B,-C serum levels and interferon-gamma (IFN-gamma) production in AR patients with pollen allergy before and after a preseasonal course of sublingual immunotherapy (SLIT). A total of 40 AR patients with pollen allergy were enrolled and given a course of preseasonal SLIT for 3 months. Serum sHLA-G and sHLA-A,-B,-C levels were determined by enzyme-linked immunoabsorbent assay, and cell production of IFN-gamma was evaluated by enzyme-linked immunoabsorbent spot assay at baseline and 3 months after the end of the SLIT course. There was a significant relationship between sHLA-G serum level change and IFN-gamma increase as well as between sHLA-A,-B,-C level change and IFN-gamma increase after SLIT. The present study provides the first published evidence that the reduction of sHLA molecules serum levels and the increased IFN-gamma production after SLIT in AR patients with pollen allergy are significantly related phenomena.
变应性鼻炎（AR）的特征是辅助性T（Th）2极化免疫反应。可溶性人类白细胞抗原（sHLA）分子发挥免疫调节作用。特异性免疫疗法是AR的唯一病因治疗方法，能够将免疫反应转变为Th1极化。本研究的目的是评估在花粉过敏的AR患者进行季节性舌下免疫疗法（SLIT）疗程前后，sHLA-G与sHLA-A、-B、-C血清水平以及干扰素-γ（IFN-γ）产生之间的关系。共纳入40例花粉过敏的AR患者，并给予为期3个月的季节性SLIT疗程。通过酶联免疫吸附测定法测定血清sHLA-G和sHLA-A、-B、-C水平，并在基线和SLIT疗程结束后3个月通过酶联免疫斑点测定法评估IFN-γ的细胞产生情况。SLIT后，sHLA-G血清水平变化与IFN-γ增加之间以及sHLA-A、-B、-C水平变化与IFN-γ增加之间存在显著关系。本研究提供了首个已发表的证据，表明花粉过敏的AR患者在SLIT后sHLA分子血清水平降低与IFN-γ产生增加是显著相关的现象。
