从聚丙烯酰胺或琼脂糖凝胶中进行大分子的电泳洗脱。

Electrophoretic elution of macromolecules from polyacrylamide or agarose gels.

作者信息

Atmeh R F, Abu-Harfiel N M, el-Sukhon S N, Abo-Shehada M N

机构信息

Department of Allied Health Sciences, University of Science and Technology, Irbid, Jordan.

出版信息

Prep Biochem. 1991;21(1):1-9. doi: 10.1080/10826069108021511.

DOI:10.1080/10826069108021511

PMID:1857680

Abstract

A method for elution of micrograms of macromolecules from polyacrylamide and agarose gels is described. The recoveries were greater than 90% with three different macromolecules tested (28 to 360 kDa). An amount as small as 1 microgram of human serum albumin was eluted from polyacrylamide gel with 90% recovery. The eluted material is collected into a small chamber the size of which can be changed as required. Elution and concentration are achieved simultaneously and in one step under mild conditions. Sterile eluates can be obtained, if the apparatus is constructed under sterile conditions.

摘要

本文描述了一种从聚丙烯酰胺和琼脂糖凝胶中洗脱微克级大分子的方法。对三种不同的大分子（28至360 kDa）进行测试，回收率均大于90%。从聚丙烯酰胺凝胶中洗脱低至1微克的人血清白蛋白，回收率达90%。洗脱后的物质收集到一个小腔室中，其大小可根据需要改变。洗脱和浓缩在温和条件下同时一步完成。如果该装置在无菌条件下构建，则可获得无菌洗脱液。