通过基于序列的高分辨率分型鉴定东非人群中的新型人类白细胞抗原G等位基因。

Identification of novel human leukocyte antigen G alleles in an East African population by high-resolution sequence-based typing.

作者信息

Turk William J R, Mao Xiao-Juan, Bielawny Thomas, Brunham Robert, Luo Ma, Plummer Francis A

机构信息

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; National Microbiology Laboratory, Winnipeg, Manitoba, Canada.

出版信息

Hum Immunol. 2008 Jul;69(7):426-9. doi: 10.1016/j.humimm.2008.05.007. Epub 2008 Jun 23.

DOI:10.1016/j.humimm.2008.05.007

PMID:18577408

Abstract

We report two novel human leukocyte antigen G (HLA-G) alleles identified in an East African population during sequence-based typing of HLA-G. The novel alleles were confirmed by sequencing multiple polymerase chain reaction products and molecular cloning and subsequent sequencing of multiple clones. The sequence of HLA-G0110 (EU290672) is identical to G01010101/01010102/01010103/01010104/01010105 at exons 2, 3, and 4 except for a single nucleotide difference at codon 31 (ACG --> ATG), resulting in a coding change from threonine to methionine. The sequence of HLA-G0111 (EU290673) is identical to G010404 at exons 2, 3, and 4 except for a single nucleotide difference at codon 31 (ACG --> ATG), resulting in a coding change from threonine to methionine. These new alleles are detected in several other individuals in our study population and the functional relevance of these new alleles must be studied.

摘要

我们报告了在基于序列的人类白细胞抗原G（HLA - G）分型过程中，于东非人群中鉴定出的两个新型HLA - G等位基因。通过对多个聚合酶链反应产物进行测序、分子克隆以及随后对多个克隆进行测序，证实了这些新型等位基因。HLA - G0110（EU290672）在外显子2、3和4的序列与G01010101/01010102/01010103/01010104/01010105相同，只是在密码子31处有一个单核苷酸差异（ACG --> ATG），导致编码从苏氨酸变为甲硫氨酸。HLA - G0111（EU290673）在外显子2、3和4的序列与G010404相同，只是在密码子31处有一个单核苷酸差异（ACG --> ATG），导致编码从苏氨酸变为甲硫氨酸。在我们的研究人群中的其他几个个体中检测到了这些新等位基因，必须对这些新等位基因的功能相关性进行研究。