在一个东非队列中鉴定出三个新型人类白细胞抗原等位基因，即HLA - B58:43、HLA - C03:190和HLA - DPA1*01:12。

Identification of three novel human leukocyte antigen alleles, HLA-B58:43, HLA-C03:190, and HLA-DPA1*01:12, in an East African cohort.

作者信息

Nykoluk M, Bailey R C, Moses S, Plummer F A, Luo M

机构信息

HIV and Human Genetics, National Microbiology Laboratory, Winnipeg, Canada.

出版信息

Tissue Antigens. 2013 Aug;82(2):131-3. doi: 10.1111/tan.12157.

DOI:10.1111/tan.12157

PMID:23849069

Abstract

Three novel human leukocyte antigen (HLA) alleles were identified using a sequence-based typing of HLA class I and class II alleles of 1867 participants from a male circumcision cohort in Kenya. The new alleles were first identified by sequencing and then confirmed by cloning the polymerase chain reaction (PCR) products and sequencing multiple clones. HLA-B58:43 was identical to HLA-B58:02 with the exception of a nucleotide change at codon 125 in exon 3 (GCC→ACC), and resulted in the amino acid change from Alanine to Threonine. HLA-C03:190 was identical to HLA-C03:02:01 with the exception of a nucleotide change at codon 131 in exon 3 (CGC→TGC), and resulted in the amino acid change from Arginine to Cysteine. HLA-DPA101:12 was identical to HLA-DPA101:03:01:01 with the exception of a nucleotide change at codon 66 in exon 2 (TTG→TCG), and resulted in the amino acid change from Leucine to Serine.

摘要

通过对肯尼亚一个男性包皮环切术队列中1867名参与者的人类白细胞抗原（HLA）I类和II类等位基因进行基于序列的分型，鉴定出三个新的HLA等位基因。这些新等位基因首先通过测序鉴定，然后通过克隆聚合酶链反应（PCR）产物并对多个克隆进行测序来确认。HLA - B58:43与HLA - B58:02相同，只是外显子3中第125位密码子处有一个核苷酸变化（GCC→ACC），导致氨基酸从丙氨酸变为苏氨酸。HLA - C03:190与HLA - C03:02:01:01相同，只是外显子3中第131位密码子处有一个核苷酸变化（CGC→TGC），导致氨基酸从精氨酸变为半胱氨酸。HLA - DPA101:12与HLA - DPA101:03:01:01:01相同，只是外显子2中第66位密码子处有一个核苷酸变化（TTG→TCG），导致氨基酸从亮氨酸变为丝氨酸。