Diao Jian, Li Shuxuan, Ma Ling, Zhang Ping, Bai Jianyang, Wang Jiaqi, Ma Xiaoqian, Ma Wei
College of Forestry, Northeast Forestry University, Harbin, China.
Institute of Forest Protection, Heilongjiang Academy of Forestry, Harbin, China.
Front Genet. 2021 Oct 22;12:769888. doi: 10.3389/fgene.2021.769888. eCollection 2021.
The major facilitator superfamily (MFS) is one of the largest known membrane transporter families. MFSs are involved in many essential functions, but studies on the MFS family in poplar have not yet been reported. Here, we identified 41 genes from (). We built a phylogenetic tree, which clearly divided members of PtrMFS into six groups with specific gene structures and protein motifs/domains. The promoter regions contain various cis-acting elements involved in stress and hormone responsiveness. Genes derived from segmental duplication events are unevenly distributed in 17 poplar chromosomes. Collinearity analysis showed that genes are conserved and homologous to corresponding genes from four other species. Transcriptome data indicated that 40 poplar genes were differentially expressed when treated with . Co-expression networks and gene function annotations of genes showed that genes tightly co-regulated and closely related in function of transmembrane transport. Taken together, we systematically analyzed structure and function of genes and proteins in the PtrMFS family. Evidence indicated that poplar genes play key roles in plant development and response to a biological stressor.
主要易化子超家族（MFS）是已知最大的膜转运蛋白家族之一。MFS参与许多重要功能，但杨树中MFS家族的研究尚未见报道。在此，我们从（）中鉴定出41个基因。我们构建了系统发育树，该树将PtrMFS成员清晰地分为六组，具有特定的基因结构和蛋白质基序/结构域。启动子区域包含参与应激和激素反应的各种顺式作用元件。来自片段重复事件的基因不均匀地分布在17条杨树染色体上。共线性分析表明，这些基因与其他四个物种的相应基因保守且同源。转录组数据表明，40个杨树基因在用（）处理时差异表达。基因的共表达网络和基因功能注释表明，这些基因紧密共调控且在跨膜运输功能上密切相关。综上所述，我们系统地分析了PtrMFS家族中基因和蛋白质的结构与功能。证据表明，杨树基因在植物发育和对生物胁迫的反应中起关键作用。
