Liu Jihua, Wang Jingrong, Yu Boyang
Department of Traditional Chinese Prescription, School of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
Se Pu. 2008 Mar;26(2):186-8.
A reversed-phase high performance liquid chromatographic method for the quantitative determination of three bufogenins in toad venom was established. An Alltech Alltima C18 column (250 mm x 4.6 mm, 5 microm) was used. The mobile phase was acetonitrile-0.1 mol/L acetate buffer (pH 3.2) (50:50, v/v), and the flow rate was 0.8 mL/min. The detection wavelength was set at 299 nm. The method has good linearity in the ranges of 0. 25 -0.875 microg for cinobufagin (r = 0.999 0), 0.25 -0.875 microg for resibufogenin (r = 0.999 1), and 0.15 - 0.525 microg for bufalin (r = 0. 999 0). The average recoveries were 100.3%, 100.0% and 98.0% for cinobufagin, resibufogenin, and bufalin, respectively. The results indicate that the method is simple, accurate, reproducible, and can be used for the quality control of bufogenins in toad venom.
建立了一种反相高效液相色谱法用于定量测定蟾酥中三种蟾毒配基。采用Alltech Alltima C18柱（250 mm×4.6 mm，5μm）。流动相为乙腈-0.1 mol/L醋酸盐缓冲液（pH 3.2）（50:50，v/v），流速为0.8 mL/min。检测波长设定为299 nm。该方法在华蟾酥毒基0.25 - 0.875μg（r = 0.999 0）、脂蟾毒配基0.25 - 0.875μg（r = 0.999 1）和蟾毒灵0.15 - 0.525μg（r = 0.999 0）范围内具有良好的线性关系。华蟾酥毒基、脂蟾毒配基和蟾毒灵的平均回收率分别为100.3%、100.0%和98.0%。结果表明该方法简便、准确、重现性好，可用于蟾酥中蟾毒配基的质量控制。
