Niscigorska-Olsen Jolanta, Wodecka Beata, Moranska Izabela, Skotarczak Bogumila
Department of Infectious Diseases and Hepatology, Pomeranian Medical Academy, Szczecin, Poland.
Ann Agric Environ Med. 2008;15(1):167-70.
Borrelia burgdorferi sensu lato (s.l.) complex, the etiological factor of Lyme disease, includes over a dozen species of bacteria and 3 pathogenic within it. According to many authors, the clinical symptoms of borreliosis depend on the species that cause the disease. The most frequent symptom of early localized borreliosis is erythema migrans (EM). The aim of the research was to determine species of B. burgdorferi s.l. in 32 patients from the Western Pomerania region in whom EM has been recognized. Blood samples of patients were investigated by PCR-RFLP method, with the use of enzyme differentiating species. The DNA of spirochetes was detected in 25 patients (25/32, 78.1%), compared with 23/32 (71.8%) of ELISA positive patients. Among 25 positive samples, 10 contained the DNA of B. garinii (10/25, 40%), 5 the DNA of B. afzelii (5/25, 20%), 4 the DNA of B. burgdorferi sensu stricto (s.s.) (4/25, 16%) and in 6 samples (6/25, 24%) the DNA of both B. garinii and B. afzelii was found. The DNA of B. burgdorferi s.l. spirochetes may be detected in patients with EM after antibiotic treatment. The most frequent species in patients with EM from the Western Pomerania region is B. garinii. Infections with more than one species of B. burgdorferi s.l. may occur in patients with EM.
莱姆病的病原体伯氏疏螺旋体狭义复合群（Borrelia burgdorferi sensu lato，s.l.）包含十几种细菌，其中有3种具有致病性。许多作者认为，莱姆病的临床症状取决于引发该病的细菌种类。早期局限性莱姆病最常见的症状是游走性红斑（EM）。本研究的目的是确定西波美拉尼亚地区32例已确诊为EM的患者体内的伯氏疏螺旋体狭义复合群种类。采用PCR-RFLP方法并使用能区分细菌种类的酶对患者的血液样本进行检测。在25例患者（25/32，78.1%）中检测到了螺旋体DNA，而ELISA检测呈阳性的患者有23/32（71.8%）。在25份阳性样本中，10份含有伽氏疏螺旋体（B. garinii）的DNA（10/25，40%），5份含有阿氏疏螺旋体（B. afzelii）的DNA（5/25，20%），4份含有狭义伯氏疏螺旋体（B. burgdorferi sensu stricto，s.s.）的DNA（4/25，16%），6份样本（6/25，24%）中同时检测到了伽氏疏螺旋体和阿氏疏螺旋体的DNA。抗生素治疗后，EM患者体内仍可检测到伯氏疏螺旋体狭义复合群螺旋体的DNA。西波美拉尼亚地区EM患者中最常见的细菌种类是伽氏疏螺旋体。EM患者可能感染不止一种伯氏疏螺旋体狭义复合群细菌。
