Lünemann J D, Zarmas S, Priem S, Franz J, Zschenderlein R, Aberer E, Klein R, Schouls L, Burmester G R, Krause A
Department of Medicine, Rheumatology and Clinical Immunology, Charité University Hospital, 10098 Berlin, Germany.
J Clin Microbiol. 2001 Mar;39(3):1130-3. doi: 10.1128/JCM.39.3.1130-1133.2001.
To further investigate the pathogenic potential of different Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were analyzed by PCR and reverse line blotting (RLB). In samples from Lyme arthritis patients, B. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, Borrelia garinii and Borrelia afzelii, respectively, were exclusively detected. The results demonstrate that PCR-RLB is a valuable tool for epidemiological and pathogenetic studies of Lyme borreliosis.
为进一步研究不同伯氏疏螺旋体基因种的致病潜力，采用聚合酶链反应（PCR）和反向线杂交（RLB）技术对27例莱姆病不同临床表现患者的标本进行了分析。在莱姆关节炎患者的样本中，主要鉴定出狭义伯氏疏螺旋体，而在神经莱姆病或肢端皮炎患者中，分别仅检测到伽氏疏螺旋体和阿氏疏螺旋体。结果表明，PCR-RLB是莱姆病流行病学和发病机制研究的一种有价值的工具。
